Detailed information of TA system TA242863

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4439993..4440509
• Replicon: chromosome
• Accession: NZ_CP096201
• Organism: Kluyvera ascorbata strain SK

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: MY052_RS21185
• Protein ID: WP_202216672.1
• Coordinates: 4439993..4440277 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A378GS61
• Locus tag: MY052_RS21190
• Protein ID: WP_035892763.1
• Coordinates: 4440267..4440509 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MY052_RS21165 (MY052_21165)	4434995..4436638	+	1644	WP_248537823.1	alpha,alpha-phosphotrehalase	-
MY052_RS21170 (MY052_21170)	4437035..4439173	+	2139	WP_248537824.1	anaerobic ribonucleoside-triphosphate reductase	-
MY052_RS21175 (MY052_21175)	4439210..4439524	+	315	WP_248537825.1	hypothetical protein	-
MY052_RS21180 (MY052_21180)	4439525..4439989	+	465	WP_035892766.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
MY052_RS21185 (MY052_21185)	4439993..4440277	-	285	WP_202216672.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MY052_RS21190 (MY052_21190)	4440267..4440509	-	243	WP_035892763.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
MY052_RS21195 (MY052_21195)	4440587..4442497	-	1911	WP_248537826.1	PRD domain-containing protein	-
MY052_RS21200 (MY052_21200)	4442520..4443671	-	1152	WP_248537827.1	lactonase family protein	-
MY052_RS21205 (MY052_21205)	4443807..4444547	-	741	WP_035892759.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-70)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11118.96 Da
• Isoelectric Point: 10.4047

>T242863 WP_202216672.1 NZ_CP096201:c4440277-4439993 [Kluyvera ascorbata]
MTYELEFDPRALREWHKLGETVKAQFKSRLAKVLENPRIPSARLHEFPDCYKIKLKSSGYRLVYQVRDDVVVVFVIAVGK
RERSAVYRDAHKRL

Nucleotide

• Length: 285 bp

>T242863 NZ_OW967966:c3769872-3769570 [Escherichia coli]
ATGGCTCAAAAGAATAATATAAGGAGTTTTCGTGATGCCTGGCTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTGTCTGGGAAATTACAGGAATATTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATATTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8987.34 Da
• Isoelectric Point: 4.6017

>AT242863 WP_035892763.1 NZ_CP096201:c4440509-4440267 [Kluyvera ascorbata]
MATLNVRLDDNLKKEAYAVLEKLDITPTEAVRLLFQYVAENGRMPVKTVTISDSEEALLQSVRERLANPEKGIKVSLDDL

Nucleotide

• Length: 243 bp

>AT242863 NZ_OW967966:c3769534-3769193 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTACTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAATAATCGTAAACTCACCACTG
AAATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A378GS61