Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3764650..3765268 Replicon chromosome
Accession NZ_CP096201
Organism Kluyvera ascorbata strain SK

Toxin (Protein)


Gene name Hha Uniprot ID A0A378GSG9
Locus tag MY052_RS18095 Protein ID WP_035891858.1
Coordinates 3765050..3765268 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A378GNK9
Locus tag MY052_RS18090 Protein ID WP_035891857.1
Coordinates 3764650..3765024 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MY052_RS18080 (MY052_18080) 3759766..3760962 + 1197 WP_123650031.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
MY052_RS18085 (MY052_18085) 3760985..3764131 + 3147 WP_115074566.1 multidrug efflux RND transporter permease subunit AcrB -
MY052_RS18090 (MY052_18090) 3764650..3765024 + 375 WP_035891857.1 Hha toxicity modulator TomB Antitoxin
MY052_RS18095 (MY052_18095) 3765050..3765268 + 219 WP_035891858.1 HHA domain-containing protein Toxin
MY052_RS18100 (MY052_18100) 3765407..3765961 + 555 WP_248537571.1 maltose O-acetyltransferase -
MY052_RS18105 (MY052_18105) 3766067..3766537 + 471 WP_248537572.1 YlaC family protein -
MY052_RS18110 (MY052_18110) 3766506..3767963 - 1458 WP_248537573.1 PLP-dependent aminotransferase family protein -
MY052_RS18115 (MY052_18115) 3768068..3768778 + 711 WP_248537574.1 GNAT family protein -
MY052_RS18120 (MY052_18120) 3768775..3768915 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
MY052_RS18125 (MY052_18125) 3768915..3769178 - 264 WP_035891863.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-115)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8613.97 Da        Isoelectric Point: 8.9008

>T242862 WP_035891858.1 NZ_CP096201:3765050-3765268 [Kluyvera ascorbata]
MSDKPLTKTDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSTVWKFIR

Download         Length: 219 bp

>T242862 NZ_OW967966:3352992-3353378 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGTAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14399.21 Da        Isoelectric Point: 4.8886

>AT242862 WP_035891857.1 NZ_CP096201:3764650-3765024 [Kluyvera ascorbata]
MDEYSPKRHDIAQLKFLCETLYHDCLVTLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYDEDNKLIAQIDEYL
DDTFMLFSNYGINSSDLQKWRKSGNKLFRCFVNASRANPVSLSC

Download         Length: 375 bp

>AT242862 NZ_OW967966:3353368-3353697 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378GSG9

Antitoxin

Source ID Structure
AlphaFold DB A0A378GNK9

References