Detailed information of TA system TA242861

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 3387627..3388333
• Replicon: chromosome
• Accession: NZ_CP096201
• Organism: Kluyvera ascorbata strain SK

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: MY052_RS16185
• Protein ID: WP_248537412.1
• Coordinates: 3387627..3388007 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: MY052_RS16190
• Protein ID: WP_202216168.1
• Coordinates: 3388004..3388333 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MY052_RS16160 (MY052_16160)	3382735..3383355	+	621	WP_035894497.1	glutathione S-transferase family protein	-
MY052_RS16165 (MY052_16165)	3383361..3384371	-	1011	WP_248537408.1	LacI family DNA-binding transcriptional regulator	-
MY052_RS16170 (MY052_16170)	3384515..3385594	+	1080	WP_248537409.1	cellulase family glycosylhydrolase	-
MY052_RS16175 (MY052_16175)	3385619..3386905	+	1287	WP_248537410.1	ABC transporter substrate-binding protein	-
MY052_RS16180 (MY052_16180)	3386947..3387330	-	384	WP_248537411.1	biofilm formation regulator BssR	-
MY052_RS16185 (MY052_16185)	3387627..3388007	+	381	WP_248537412.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MY052_RS16190 (MY052_16190)	3388004..3388333	+	330	WP_202216168.1	helix-turn-helix domain-containing protein	Antitoxin
MY052_RS16195 (MY052_16195)	3388383..3389294	-	912	WP_248537413.1	glutathione ABC transporter permease GsiD	-
MY052_RS16200 (MY052_16200)	3389296..3390216	-	921	WP_123651360.1	glutathione ABC transporter permease GsiC	-
MY052_RS16205 (MY052_16205)	3390269..3391807	-	1539	WP_248537414.1	glutathione ABC transporter substrate-binding protein GsiB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14118.30 Da
• Isoelectric Point: 10.0042

>T242861 WP_248537412.1 NZ_CP096201:3387627-3388007 [Kluyvera ascorbata]
MTIYKAKRFNQNTKKLGITDDVLLKAAEDIKQGVWEANLGGGVIKKRLPLQQGKSGGARTVIFFKYDRHIFFYDGWAKNS
VSSGNGKEIEDDELIAYRKLASAFLAFTDKDIAKLLITGFLVEVDK

Nucleotide

• Length: 381 bp

>T242861 NZ_OW967966:3053641-3054135 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12034.19 Da
• Isoelectric Point: 10.5858

>AT242861 WP_202216168.1 NZ_CP096201:3388004-3388333 [Kluyvera ascorbata]
MTNKLKTILDDAKELNALGLMPENDVAEINKLLKARELKTRIAAVKMMSGEEIKAVRARFGMSQSILAHTLGMSKESVSK
WERNEIKPSGPALRILNTLAAKGPEVFTK

Nucleotide

• Length: 330 bp

>AT242861 NZ_OW967966:3053351-3053644 [Escherichia coli]
ATGGCAGCAGCCAATAACATTCCAGCCAGGCGCGAATCTTTAAACTTGCGCATAAAACCCGCAGAACGCGATCTTATAGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCTCCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA