Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 320503..321089 Replicon chromosome
Accession NZ_CP096201
Organism Kluyvera ascorbata strain SK

Toxin (Protein)


Gene name doc Uniprot ID A0A378GI35
Locus tag MY052_RS01465 Protein ID WP_035892171.1
Coordinates 320721..321089 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A378GGU8
Locus tag MY052_RS01460 Protein ID WP_035892172.1
Coordinates 320503..320724 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MY052_RS01435 (MY052_01435) 315520..316629 + 1110 WP_035892180.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
MY052_RS01440 (MY052_01440) 316684..317610 + 927 WP_035892178.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
MY052_RS01445 (MY052_01445) 317607..318887 + 1281 WP_035892176.1 branched chain amino acid ABC transporter permease LivM -
MY052_RS01450 (MY052_01450) 318884..319651 + 768 WP_035892174.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
MY052_RS01455 (MY052_01455) 319653..320366 + 714 WP_081918523.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
MY052_RS01460 (MY052_01460) 320503..320724 + 222 WP_035892172.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
MY052_RS01465 (MY052_01465) 320721..321089 + 369 WP_035892171.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
MY052_RS01470 (MY052_01470) 321350..322666 + 1317 WP_035892169.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
MY052_RS01475 (MY052_01475) 322767..323654 + 888 WP_248538103.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
MY052_RS01480 (MY052_01480) 323651..324496 + 846 WP_035892165.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
MY052_RS01485 (MY052_01485) 324499..325569 + 1071 WP_248538104.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 317607..326306 8699


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(1-121)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13501.79 Da        Isoelectric Point: 7.3179

>T242845 WP_035892171.1 NZ_CP096201:320721-321089 [Kluyvera ascorbata]
MNLQIISAEEIIAFHDRLLRVTPGVVGMPDPGRAEALMYRVLNRIEYEGVTDVWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGITLAANPDYVAMTVEAAAGQLTLEQIASRLRG

Download         Length: 369 bp

>T242845 NZ_OW967966:223368-223553 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8157.18 Da        Isoelectric Point: 4.5938

>AT242845 WP_035892172.1 NZ_CP096201:320503-320724 [Kluyvera ascorbata]
MRTINYSEARQNLAEVLESAVTGVPVTITRRGHKPAVIISAEEFERYQAARLDDEFAAIMAIHGNEIEELADK

Download         Length: 222 bp

>AT242845 NZ_OW967966:223570-223980 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGCCGCGCACTTCGAAACCTTATGCGAGATGG
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGCGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCGAAAAGGTTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTACGCCAACAGAAGTGCGTTTCTGGCAGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378GI35

Antitoxin

Source ID Structure
AlphaFold DB A0A378GGU8

References