Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 614071..614647 Replicon chromosome
Accession NZ_CP096187
Organism Enterobacter roggenkampii isolate C3-17

Toxin (Protein)


Gene name shpA Uniprot ID A0A656ULU9
Locus tag M0769_RS02910 Protein ID WP_025912099.1
Coordinates 614071..614358 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A156JBA1
Locus tag M0769_RS02915 Protein ID WP_023293359.1
Coordinates 614345..614647 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M0769_RS02880 (M0769_02880) 609082..609198 + 117 Protein_558 amino acid-binding protein -
M0769_RS02885 (M0769_02885) 609191..609886 + 696 WP_045335574.1 winged helix-turn-helix domain-containing protein -
M0769_RS02890 (M0769_02890) 610033..610503 + 471 WP_008501325.1 MarR family transcriptional regulator -
M0769_RS02895 (M0769_02895) 610500..611567 + 1068 WP_025912094.1 HlyD family secretion protein -
M0769_RS02900 (M0769_02900) 611557..612633 + 1077 WP_045335580.1 DUF2955 domain-containing protein -
M0769_RS02905 (M0769_02905) 612630..613901 - 1272 WP_181530822.1 DUF445 domain-containing protein -
M0769_RS02910 (M0769_02910) 614071..614358 + 288 WP_025912099.1 BrnT family toxin Toxin
M0769_RS02915 (M0769_02915) 614345..614647 + 303 WP_023293359.1 BrnA antitoxin family protein Antitoxin
M0769_RS02920 (M0769_02920) 614678..615316 - 639 WP_161622970.1 LysE family translocator -
M0769_RS02925 (M0769_02925) 615364..616107 - 744 WP_161622971.1 AraC family transcriptional regulator -
M0769_RS02930 (M0769_02930) 616259..617629 + 1371 WP_181530820.1 NAD-dependent succinate-semialdehyde dehydrogenase -
M0769_RS02935 (M0769_02935) 617672..617992 - 321 WP_008501314.1 hypothetical protein -
M0769_RS02940 (M0769_02940) 617992..618537 - 546 WP_008501313.1 YfaZ family outer membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11235.68 Da        Isoelectric Point: 7.4007

>T242825 WP_025912099.1 NZ_CP096187:614071-614358 [Enterobacter roggenkampii]
MPTEYEWDSNKAKSNLQKHGIRFEDAVMVFDDPYHLSVQDRYENGEFRWQTIGLVQGLLVILVAHTVRFESGGEIIRIIS
ARKADRKERSRYEHR

Download         Length: 288 bp

>T242825 NZ_OW967802:769695-769991 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATACCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11369.01 Da        Isoelectric Point: 10.2483

>AT242825 WP_023293359.1 NZ_CP096187:614345-614647 [Enterobacter roggenkampii]
MSIVKHKRGSAPEVSARRVAELKALAEKPDAEIDYSDIPATDDEQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT242825 NZ_OW967802:769993-770388 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A656ULU9

Antitoxin

Source ID Structure
AlphaFold DB A0A156JBA1

References