Detailed information of TA system TA242822

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 84896..85575
• Replicon: chromosome
• Accession: NZ_CP096187
• Organism: Enterobacter roggenkampii isolate C3-17

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: M0769_RS00390
• Protein ID: WP_057061651.1
• Coordinates: 84896..85237 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: M0769_RS00395
• Protein ID: WP_057061652.1
• Coordinates: 85258..85575 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M0769_RS00365 (M0769_00365)	80507..81445	+	939	WP_123923919.1	helix-turn-helix domain-containing protein	-
M0769_RS00370 (M0769_00370)	81531..82577	+	1047	WP_181530986.1	class I SAM-dependent methyltransferase	-
M0769_RS00375 (M0769_00375)	82644..83099	+	456	WP_123923097.1	acetyltransferase	-
M0769_RS00380 (M0769_00380)	83089..83256	-	168	Protein_75	hydroxyacid dehydrogenase	-
M0769_RS00390 (M0769_00390)	84896..85237	-	342	WP_057061651.1	TA system toxin CbtA family protein	Toxin
M0769_RS00395 (M0769_00395)	85258..85575	-	318	WP_057061652.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
M0769_RS00400 (M0769_00400)	85594..85815	-	222	WP_057061653.1	DUF987 domain-containing protein	-
M0769_RS00405 (M0769_00405)	85824..86300	-	477	WP_057061654.1	RadC family protein	-
M0769_RS00410 (M0769_00410)	86316..86774	-	459	WP_057061655.1	antirestriction protein	-
M0769_RS00415 (M0769_00415)	86877..87116	-	240	WP_057061656.1	DUF905 domain-containing protein	-
M0769_RS00420 (M0769_00420)	87193..87660	-	468	WP_057061657.1	protein YkfB	-
M0769_RS00425 (M0769_00425)	87684..88127	-	444	WP_057061658.1	hypothetical protein	-
M0769_RS00430 (M0769_00430)	88127..88363	-	237	WP_032181217.1	protein YpjK	-
M0769_RS00435 (M0769_00435)	88404..89105	-	702	WP_057061659.1	WYL domain-containing protein	-
M0769_RS00440 (M0769_00440)	89322..90143	-	822	WP_057061660.1	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	84896..109642	24746
	-	flank	IS/Tn	-	-	83677..84192	515

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (5-103)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12845.82 Da
• Isoelectric Point: 9.6253

>T242822 WP_057061651.1 NZ_CP096187:c85237-84896 [Enterobacter roggenkampii]
MKSQPATTSRAVKPCLSPVAVWQMLLTRLLEKHYGLTLNDTPFSDETVIQEHINAGITMADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNVMG

Nucleotide

• Length: 342 bp

>T242822 NZ_OW967802:318955-319185 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11826.46 Da
• Isoelectric Point: 6.9365

>AT242822 WP_057061652.1 NZ_CP096187:c85575-85258 [Enterobacter roggenkampii]
MCNITWGLQRDITPRLGARLVQEGNRLHYLADRASITGKFSDAGCRKLDDTFPHFISQMESMLTMGELNPQHAHCVTLYH
NGFTCEADTLGSCGYVYITVYPTPR

Nucleotide

• Length: 318 bp

>AT242822 NZ_OW967802:319182-319541 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA