Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK(toxin)
Location 289739..290307 Replicon chromosome
Accession NZ_CP096184
Organism Demequina sp. TMPB413

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag LGT36_RS01330 Protein ID WP_226096250.1
Coordinates 289942..290307 (+) Length 122 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag LGT36_RS01325 Protein ID WP_248642142.1
Coordinates 289739..289942 (+) Length 68 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGT36_RS01300 (LGT36_001300) 285034..286332 - 1299 WP_226096270.1 3-phosphoshikimate 1-carboxyvinyltransferase -
LGT36_RS01305 (LGT36_001305) 286368..286853 - 486 WP_226096259.1 hypothetical protein -
LGT36_RS01310 (LGT36_001310) 287029..287607 + 579 WP_226096257.1 sigma-70 family RNA polymerase sigma factor -
LGT36_RS01315 (LGT36_001315) 287679..287924 + 246 WP_226096255.1 mycothiol system anti-sigma-R factor -
LGT36_RS01320 (LGT36_001320) 288353..289441 + 1089 WP_226096254.1 PilT/PilU family type 4a pilus ATPase -
LGT36_RS01325 (LGT36_001325) 289739..289942 + 204 WP_248642142.1 ribbon-helix-helix domain-containing protein Antitoxin
LGT36_RS01330 (LGT36_001330) 289942..290307 + 366 WP_226096250.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LGT36_RS01335 (LGT36_001335) 290304..290906 - 603 WP_226096249.1 GDSL-type esterase/lipase family protein -
LGT36_RS01340 (LGT36_001340) 290903..294604 - 3702 WP_226096247.1 multifunctional oxoglutarate decarboxylase/oxoglutarate dehydrogenase thiamine pyrophosphate-binding subunit/dihydrolipoyllysine-residue succinyltransferase subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-114)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-40)


Sequences

Toxin        


Download         Length: 122 a.a.        Molecular weight: 12380.17 Da        Isoelectric Point: 11.7887

>T242821 WP_226096250.1 NZ_CP096184:289942-290307 [Demequina sp. TMPB413]
MRRGEIRWVALDAGADAEARSRAQRPAVIVSNDGANTAAARRGSGAVTVVPLTTSDAPAKPFQVALAAGVGGLPRAAKAM
AEQVRTVSAGRVGPSIGMLSLSDVAKIDDALRLHLGLDRGR

Download         Length: 366 bp

>T242821 NZ_OW967802:c49719-49342 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCATCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin


Download         Length: 68 a.a.        Molecular weight: 7136.74 Da        Isoelectric Point: 4.2131

>AT242821 WP_248642142.1 NZ_CP096184:289739-289942 [Demequina sp. TMPB413]
VKLSISLTDDDVALIDQFAAAGDYPSRSAVIQHALARLRAQGLHGDYADAWTEFSDGGDAGRWEASS

Download         Length: 204 bp

>AT242821 NZ_OW967802:c50143-49766 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCATCACACTGTATGCGAAGGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGCTTATCTGGCTGTTTATCCGACACCGGCAGAGCCCGCAATCACCGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References