Detailed information of TA system TA242814

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 2904317..2904909
• Replicon: chromosome
• Accession: NZ_CP096169
• Organism: Shewanella sp. JNE2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: MZ182_RS12935
• Protein ID: WP_248069775.1
• Coordinates: 2904317..2904571 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A252ENK1
• Locus tag: MZ182_RS12940
• Protein ID: WP_014610929.1
• Coordinates: 2904568..2904909 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MZ182_RS12900 (MZ182_12900)	2899441..2900166	+	726	WP_220777342.1	zeta toxin family protein	-
MZ182_RS12905 (MZ182_12905)	2900188..2900352	+	165	WP_014610884.1	hypothetical protein	-
MZ182_RS12910 (MZ182_12910)	2900430..2900606	-	177	WP_248085063.1	tyrosine-type recombinase/integrase	-
MZ182_RS12915 (MZ182_12915)	2900678..2901691	-	1014	WP_248069778.1	dTDP-glucose 4,6-dehydratase	-
MZ182_RS12920 (MZ182_12920)	2902242..2902619	-	378	Protein_2494	transposase	-
MZ182_RS12925 (MZ182_12925)	2902824..2903312	+	489	WP_069453178.1	mobile mystery protein A	-
MZ182_RS12930 (MZ182_12930)	2903309..2903920	+	612	WP_248069777.1	mobile mystery protein B	-
MZ182_RS12935 (MZ182_12935)	2904317..2904571	+	255	WP_248069775.1	type II toxin-antitoxin system HicA family toxin	Toxin
MZ182_RS12940 (MZ182_12940)	2904568..2904909	+	342	WP_014610929.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
MZ182_RS12945 (MZ182_12945)	2905072..2905452	-	381	WP_248069773.1	helix-turn-helix transcriptional regulator	-
MZ182_RS12950 (MZ182_12950)	2905449..2906816	-	1368	WP_248069771.1	HipA domain-containing protein	-
MZ182_RS12955 (MZ182_12955)	2907171..2907488	-	318	WP_014610892.1	CcdB family protein	-
MZ182_RS12960 (MZ182_12960)	2907488..2907733	-	246	WP_014610893.1	type II toxin-antitoxin system CcdA family antitoxin	-
MZ182_RS12965 (MZ182_12965)	2908044..2908136	+	93	Protein_2503	transposase family protein	-
MZ182_RS12970 (MZ182_12970)	2908974..2909240	+	267	WP_140906946.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2902242..2902691	449

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-74)

Antitoxin

• Uncharacterised protein family HicB (55-104)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9508.02 Da
• Isoelectric Point: 10.1453

>T242814 WP_248069775.1 NZ_CP096169:2904317-2904571 [Shewanella sp. JNE2]
MNKKHLRTLTAIFARPVSGAIKWSDIEALFIALGADIEEREGSRIGVVLFGEVQVYHRPHPQKETDKDAVINIKKWLERN
GVKA

Nucleotide

• Length: 255 bp

>T242814 NZ_OW967795:c3813910-3813596 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12379.91 Da
• Isoelectric Point: 4.3992

>AT242814 WP_014610929.1 NZ_CP096169:2904568-2904909 [Shewanella sp. JNE2]
MKNLMMINGVKAFIDYDPDAETFRGEFVGLNGGADFYGESVAQLEAEGAKSLSVFLEMCQERGIEPYKNFSGKFNVRISP
EVHARLNEIALSQSISLNAAVENAVNDYIAHSA

Nucleotide

• Length: 342 bp

>AT242814 NZ_OW967795:c3814146-3813913 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A252ENK1