Detailed information of TA system TA242813

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc(toxin)
• Location: 2019115..2019674
• Replicon: chromosome
• Accession: NZ_CP096169
• Organism: Shewanella sp. JNE2

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: MZ182_RS09085
• Protein ID: WP_172591132.1
• Coordinates: 2019285..2019674 (+)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: MZ182_RS09080
• Protein ID: WP_172591131.1
• Coordinates: 2019115..2019285 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MZ182_RS09065 (MZ182_09065)	2014738..2016732	+	1995	WP_011919219.1	nitrate- and nitrite sensing domain-containing protein	-
MZ182_RS09075 (MZ182_09075)	2017163..2018989	+	1827	WP_248065872.1	site-specific integrase	-
MZ182_RS09080 (MZ182_09080)	2019115..2019285	+	171	WP_172591131.1	acetyltransferase	Antitoxin
MZ182_RS09085 (MZ182_09085)	2019285..2019674	+	390	WP_172591132.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
MZ182_RS09090 (MZ182_09090)	2019799..2022525	-	2727	WP_248065871.1	phage/plasmid primase, P4 family	-
MZ182_RS09095 (MZ182_09095)	2022672..2022995	-	324	WP_248065869.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-125)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14212.28 Da
• Isoelectric Point: 3.9777

>T242813 WP_172591132.1 NZ_CP096169:2019285-2019674 [Shewanella sp. JNE2]
MDIICFPFERVVEINALILSTEPGMKGAVDIPKLQGALSRIDNAIVYQGLDDVFEIAAKYTACIAVSHACPDANKRTGLA
VALEYLSLNDYEITEDNELLANAIRDLVLQEITETDFADILYAQYLKGL

Nucleotide

• Length: 390 bp

>T242813 NZ_OW967795:c3572662-3572264 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6414.58 Da
• Isoelectric Point: 11.0174

>AT242813 WP_172591131.1 NZ_CP096169:2019115-2019285 [Shewanella sp. JNE2]
MNRKLEVYGVQVVTRPKIKATKQLDLSGDFGRQIVKSETKLALRTHPKTFQKLADM

Nucleotide

• Length: 171 bp

>AT242813 NZ_OW967795:c3572958-3572665 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA