Detailed information of TA system TA242808

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 3431057..3432779
• Replicon: chromosome
• Accession: NZ_CP096166
• Organism: Shewanella sp. JNE7

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: D0FZT9
• Locus tag: MZ097_RS15395
• Protein ID: WP_000356587.1
• Coordinates: 3431057..3431995 (-)
• Length: 313 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: MZ097_RS15400
• Protein ID: WP_048608544.1
• Coordinates: 3431988..3432779 (-)
• Length: 264 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MZ097_RS15375 (MZ097_15380)	3426998..3427510	-	513	WP_172689549.1	S26 family signal peptidase	-
MZ097_RS15380 (MZ097_15385)	3427494..3427841	-	348	WP_048608540.1	hypothetical protein	-
MZ097_RS15385 (MZ097_15390)	3427834..3430233	-	2400	WP_048608542.1	type IV secretion system protein TraC	-
MZ097_RS15390 (MZ097_15395)	3430233..3430925	-	693	WP_001228924.1	DsbC family protein	-
MZ097_RS15395 (MZ097_15400)	3431057..3431995	-	939	WP_000356587.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
MZ097_RS15400 (MZ097_15405)	3431988..3432779	-	792	WP_048608544.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
MZ097_RS15405 (MZ097_15410)	3433000..3433386	-	387	WP_000180225.1	TraA family conjugative transfer protein	-
MZ097_RS15410 (MZ097_15415)	3433383..3433955	-	573	WP_139175592.1	type IV conjugative transfer system lipoprotein TraV	-
MZ097_RS15415 (MZ097_15420)	3434030..3435319	-	1290	WP_085285719.1	TraB/VirB10 family protein	-
MZ097_RS15420 (MZ097_15425)	3435322..3436218	-	897	WP_048608549.1	type-F conjugative transfer system secretin TraK	-
MZ097_RS15425 (MZ097_15430)	3436202..3436828	-	627	WP_000667170.1	TraE/TraK family type IV conjugative transfer system protein	-
MZ097_RS15430 (MZ097_15435)	3436825..3437106	-	282	WP_000433889.1	type IV conjugative transfer system protein TraL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	sul2 / aph(3'')-Ib / aph(6)-Id / floR / erm(42) / mph(A) / sul1 / qacE / aadA2 / dfrA12 / aac(3)-IId	adeG	3286634..3496315	209681

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-97)

Sequences

Toxin

Protein

• Length: 313 a.a.
• Molecular weight: 35548.82 Da
• Isoelectric Point: 4.9081

>T242808 WP_000356587.1 NZ_CP096166:c3431995-3431057 [Shewanella sp. JNE7]
MDKDSPYYKQVSLLIRMLPVVATETVFALKGGTAINLFVRDFPRLSVDIDLAYLPLEPRDEALINVRAALQRITDRINTQ
PDIRAAFQDNKADELRIVVSSPVATIKIEVSPVARGTLHSAEIMPVQESVEDEFGYAEIQVVSLPDLYGGKLCAAMDRQH
PRDLFDVRMLLGSEGISREILVGFLTYTLSHPRPINEVMSPNWQPLNEKFQAEFDGMTFEKVECEDLASVRPMMLIELQK
HFTERDHAFLMSFKRGQPDWALFDYPNAADLPAIRWKLQNINKLAKNQAKHQEQLDKLKQVLDDWLVNANAE

Nucleotide

• Length: 939 bp

>T242808 NZ_OW967795:2327896-2329218 [Escherichia coli]
ATGCCTAAACTAGTCACTTGGATGAACAACCAGCGTGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCTGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAGTCCCCGAAGA
TGAAACCGTAACGTGTCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGTTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACTGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAGGGAATAACGCCAACGACGCACATTATCAAATTGCCGATTGGCGAAAT
CAGACAGCCCAATGCGACGCTCGATCTTAGCCAAAGCGTTGATAATGAGTATTACTGTTTGCTGCTGGCGAAAGAACTCG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGCCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCCGGTATCGCGCAAATTATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTCTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGGCTCACACCATTTTACGACATCATTTCGGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACGATCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin

Protein

• Length: 264 a.a.
• Molecular weight: 30100.44 Da
• Isoelectric Point: 9.3513

>AT242808 WP_048608544.1 NZ_CP096166:c3432779-3431988 [Shewanella sp. JNE7]
MSSKINWLVAHTSPGALVLQQWLTENGVSYSLAQKYAQNGWLKKLSSGVYYRPDAQGDIKPTWVDAIQALDVQLGVSVHL
AGLSSLTHQGLSHYLQLNKEQVWICVKNKSSLPKWFREFPYQNWFYCGNHKLEVNPEKDLKRITVKEKELTVSCAELAAY
EVVDAIGKLISFEHVAELFQGLVNLSPRKVQDILERSSSVQANRIFLFLGRYYDHQWVNRIDETRIKLGAGKRQVVEKGR
FDERYQITVPEILSVKKGEQHNG

Nucleotide

• Length: 792 bp

>AT242808 NZ_OW967795:2327630-2327896 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAACGGCTGGAC
GCAGAGCGAGTTGGCGAAAAAAATCGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAATCCTGACAATACCTCGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAAGCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: A0A1U9VZ44