Detailed information of TA system TA242805

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 3008803..3009395
• Replicon: chromosome
• Accession: NZ_CP096166
• Organism: Shewanella sp. JNE7

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: MZ097_RS13420
• Protein ID: WP_248069775.1
• Coordinates: 3008803..3009057 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A252ENK1
• Locus tag: MZ097_RS13425
• Protein ID: WP_014610929.1
• Coordinates: 3009054..3009395 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MZ097_RS13385 (MZ097_13385)	3003927..3004652	+	726	WP_220777342.1	zeta toxin family protein	-
MZ097_RS13390 (MZ097_13390)	3004674..3004838	+	165	WP_014610884.1	hypothetical protein	-
MZ097_RS13395 (MZ097_13395)	3004916..3005092	-	177	WP_248085063.1	tyrosine-type recombinase/integrase	-
MZ097_RS13400 (MZ097_13400)	3005164..3006177	-	1014	WP_248069778.1	dTDP-glucose 4,6-dehydratase	-
MZ097_RS13405 (MZ097_13405)	3006728..3007105	-	378	Protein_2595	transposase	-
MZ097_RS13410 (MZ097_13410)	3007310..3007798	+	489	WP_069453178.1	mobile mystery protein A	-
MZ097_RS13415 (MZ097_13415)	3007795..3008406	+	612	WP_248069777.1	mobile mystery protein B	-
MZ097_RS13420 (MZ097_13420)	3008803..3009057	+	255	WP_248069775.1	type II toxin-antitoxin system HicA family toxin	Toxin
MZ097_RS13425 (MZ097_13425)	3009054..3009395	+	342	WP_014610929.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
MZ097_RS13430 (MZ097_13430)	3009558..3009938	-	381	WP_248069773.1	helix-turn-helix transcriptional regulator	-
MZ097_RS13435 (MZ097_13435)	3009935..3011302	-	1368	WP_248069771.1	HipA domain-containing protein	-
MZ097_RS13440 (MZ097_13440)	3011657..3011974	-	318	WP_014610892.1	CcdB family protein	-
MZ097_RS13445 (MZ097_13445)	3011974..3012219	-	246	WP_014610893.1	type II toxin-antitoxin system CcdA family antitoxin	-
MZ097_RS13450 (MZ097_13450)	3012530..3012622	+	93	Protein_2604	transposase family protein	-
MZ097_RS13455 (MZ097_13455)	3013460..3013726	+	267	WP_140906946.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3006728..3007177	449

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-74)

Antitoxin

• Uncharacterised protein family HicB (55-104)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9508.02 Da
• Isoelectric Point: 10.1453

>T242805 WP_248069775.1 NZ_CP096166:3008803-3009057 [Shewanella sp. JNE7]
MNKKHLRTLTAIFARPVSGAIKWSDIEALFIALGADIEEREGSRIGVVLFGEVQVYHRPHPQKETDKDAVINIKKWLERN
GVKA

Nucleotide

• Length: 255 bp

>T242805 NZ_OW967795:1812308-1812562 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCAATACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12379.91 Da
• Isoelectric Point: 4.3992

>AT242805 WP_014610929.1 NZ_CP096166:3009054-3009395 [Shewanella sp. JNE7]
MKNLMMINGVKAFIDYDPDAETFRGEFVGLNGGADFYGESVAQLEAEGAKSLSVFLEMCQERGIEPYKNFSGKFNVRISP
EVHARLNEIALSQSISLNAAVENAVNDYIAHSA

Nucleotide

• Length: 342 bp

>AT242805 NZ_OW967795:1812060-1812311 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A252ENK1