Detailed information of TA system TA242804

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc(toxin)
• Location: 2050129..2050688
• Replicon: chromosome
• Accession: NZ_CP096166
• Organism: Shewanella sp. JNE7

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: MZ097_RS09215
• Protein ID: WP_172591132.1
• Coordinates: 2050299..2050688 (+)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: MZ097_RS09210
• Protein ID: WP_172591131.1
• Coordinates: 2050129..2050299 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MZ097_RS09185 (MZ097_09185)	2045753..2046433	-	681	WP_248084399.1	hypothetical protein	-
MZ097_RS09190 (MZ097_09190)	2046652..2046945	-	294	WP_248084400.1	hypothetical protein	-
MZ097_RS09195 (MZ097_09195)	2047129..2047428	-	300	Protein_1786	transposase	-
MZ097_RS09200 (MZ097_09200)	2047474..2047632	+	159	WP_248084401.1	hypothetical protein	-
MZ097_RS09205 (MZ097_09205)	2048177..2050003	+	1827	WP_248065872.1	site-specific integrase	-
MZ097_RS09210 (MZ097_09210)	2050129..2050299	+	171	WP_172591131.1	acetyltransferase	Antitoxin
MZ097_RS09215 (MZ097_09215)	2050299..2050688	+	390	WP_172591132.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
MZ097_RS09220 (MZ097_09220)	2050813..2053539	-	2727	WP_248065871.1	phage/plasmid primase, P4 family	-
MZ097_RS09225 (MZ097_09225)	2053686..2054009	-	324	WP_248084402.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-125)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14212.28 Da
• Isoelectric Point: 3.9777

>T242804 WP_172591132.1 NZ_CP096166:2050299-2050688 [Shewanella sp. JNE7]
MDIICFPFERVVEINALILSTEPGMKGAVDIPKLQGALSRIDNAIVYQGLDDVFEIAAKYTACIAVSHACPDANKRTGLA
VALEYLSLNDYEITEDNELLANAIRDLVLQEITETDFADILYAQYLKGL

Nucleotide

• Length: 390 bp

>T242804 NZ_OW967795:1812060-1812311 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6414.58 Da
• Isoelectric Point: 11.0174

>AT242804 WP_172591131.1 NZ_CP096166:2050129-2050299 [Shewanella sp. JNE7]
MNRKLEVYGVQVVTRPKIKATKQLDLSGDFGRQIVKSETKLALRTHPKTFQKLADM

Nucleotide

• Length: 171 bp

>AT242804 NZ_OW967795:1812308-1812562 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCAATACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA