Detailed information of TA system TA242790

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 24657..25234
• Replicon: plasmid unnamed
• Accession: NZ_CP096160
• Organism: Listeria monocytogenes strain FSL J1-0049

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: H1G8D6
• Locus tag: MZN42_RS15210
• Protein ID: WP_003728465.1
• Coordinates: 24893..25234 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H1G8D5
• Locus tag: MZN42_RS15205
• Protein ID: WP_003728464.1
• Coordinates: 24657..24899 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MZN42_RS15160 (MZN42_15155)	19929..20645	-	717	WP_003731678.1	AP2 domain-containing protein	-
MZN42_RS15165 (MZN42_15160)	20661..21383	-	723	WP_003728517.1	CRISPR-associated protein Cas5	-
MZN42_RS15170 (MZN42_15165)	21387..21812	-	426	WP_003728518.1	hypothetical protein	-
MZN42_RS15175 (MZN42_15170)	21813..22040	-	228	WP_003769261.1	hypothetical protein	-
MZN42_RS15180 (MZN42_15175)	22183..22431	-	249	WP_023553717.1	hypothetical protein	-
MZN42_RS15185 (MZN42_15180)	22830..23207	-	378	WP_003769263.1	DUF3850 domain-containing protein	-
MZN42_RS15190 (MZN42_15185)	23226..23888	-	663	WP_003731676.1	DUF2786 domain-containing protein	-
MZN42_RS15195 (MZN42_15190)	23888..24214	-	327	WP_003728462.1	hypothetical protein	-
MZN42_RS15200 (MZN42_15195)	24360..24596	+	237	WP_003769265.1	hypothetical protein	-
MZN42_RS15205 (MZN42_15200)	24657..24899	+	243	WP_003728464.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
MZN42_RS15210 (MZN42_15205)	24893..25234	+	342	WP_003728465.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MZN42_RS15215 (MZN42_15210)	25427..27562	-	2136	WP_003728466.1	heavy metal translocating P-type ATPase	-
MZN42_RS15220 (MZN42_15215)	27562..27921	-	360	WP_003728467.1	Cd(II)-sensing metalloregulatory transcriptional repressor CadC	-
MZN42_RS15225 (MZN42_15220)	28201..28755	+	555	WP_003728468.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	ClpL	-	1..57553	57553

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-105)

Antitoxin

• SpoVT-AbrB domain (7-46)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12827.74 Da
• Isoelectric Point: 9.2830

>T242790 WP_003728465.1 NZ_CP096160:24893-25234 [Listeria monocytogenes]
MVKQGDIIKINLNPKQGHEQQGYRPYICLSHHLVSDYANIAIFAPISNTPRTYPLYVPLTGTTTTGKVLLDQLVTIDYNA
RKHQYVETVSDPLLTKLLDTVKVIFQKNERSTN

Nucleotide

• Length: 342 bp

>T242790 NZ_OW967389:107642-108967 [Enterobacter cloacae]
ATGAGCCGGAAACAGCAGCGTCTGGCAATCTGGATGAACGGAATCAAAGTCGGATACTGGGAAAAAAGTAAAGGGGTAGA
CAGCTTACAATACCTTCCTGACTGGAGTGCAGACGAACAGGGAAGGCCGCTGTCATTGTCCCTTCCTTTTACTCCCGGGA
ACCAGGTCTGGCGTGGAAATGTGGTACGTGACTATTTTGATAATTTACTCCCGGACAGCGAAGGCATACGCAGACGTCTG
GCAATGCGTTACAAGGCCGATAGCCTGGAGCCTTTTGATCTTCTGACGGAGCTGGGAAAAGACTGCGTGGGCGCGATACA
GCTGCTTCATGACGGAGATGAACCCACAGATTTATATTCCGTGAAGTATCACCCGCTTACTGAATCAGAAATTGCGGCAA
CTTTGCGTAATACCACGGAGACATTGCTACCGGGCAGGCCAGAAGATAACGACGACTTACGTTTATCTATTGCCGGTGCT
CAGGAGAAAACAGCCCTACTGTGGCATGAAGATCGGTGGTGTATGCCGGAGGGTAATACACCAACGACGCATATATTCAA
GCTACCGCTCGGACTTGTGGGGAACATGAAAGCCGACATGCGCTCGTCGGTTGAAAATGAATGGCTCTGCTCGGTACTTC
TCGAACAGTACGGGCTCCCCGTGGCAAGAACGCAGATTGCTCACTTTGAAGATCAGAAAGCGCTGGTGGTAGAACGTTTT
GACCGAAAATGGTCAGGAAACGGACAATGGATCATTCGTTTACCCCAGGAGGATATGTGTCAGGCCCTGGGCATTTCGCC
ATTACGAAAATATCAGGCGGACGGCGGGCCAGGTATATCGGAAATTATGGAAGTTCTGAGTAATTCAGACCGTGCTGAGC
GTGACAAAGCGCAGTTTTTTATGACGCAGATAGTTTTCTGGATGATGGCCGCAACAGATGGCCACGCTAAAAATTTCAGT
ATTTCTATTGGGCCACAGGGACGTTATCACCTTACACCACTCTATGATGTGTTGTCGGCATGGCCGGTGATCGGTCATGG
GAACAATCAGATATCCTGGCAGAAGTGCAAACTGGCGATGGCCGTACGTGGCAGTAGCAACTATTACCAGATATATCGAA
TACAACGACGCCACTGGATCAGGCATGGTGAGATAACCGGCCTGAGTAAACAGCAGACAGAGGCGATGATAGAGGAAATC
ATCGCCAGAACCCCGGGTGTCATTGAACGTGTTAGCGGGCTTCTTCCGGACCAGTTCCCCCAGCAGCTTGCGGAAAGTAT
TTTTGATGGAATGAGGCAGCAATGCAGGCGCCTGGCTGAGAAATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9207.48 Da
• Isoelectric Point: 4.3867

>AT242790 WP_003728464.1 NZ_CP096160:24657-24899 [Listeria monocytogenes]
MTTVSVKKWGNSKAVRLPNNLLNALDIKENDDLKVEVINNQMILTKTAIDLRIEDLFANYQGEPFQTELQTFEPIGDEKW

Nucleotide

• Length: 243 bp

>AT242790 NZ_OW967389:107344-107637 [Enterobacter cloacae]
ATCAATAATGATTACCCGTTAAACACGCTCAACCAGTTGCGTCCCTTGCTCATTGGTTTTCGCAAGGCGAATGGCCTGAC
GCAAAAAGACCTGTCCGAAAGGTTAGGGGTCACTCAACAGACATACTCACGTCTGGAAGCCAACCCTGCCAGTGCGAGCA
TTGAACGGCTATTCAAGGTGTTTTCTATCCTGGGCGTAAAAATCAGCTTCTCCTCGACAACCGCCTCTTCAGAGGGGAAG
CAGACGGAAGAAATGCTTAAATCAAACTCACCTGCACGACAGGAGAAATGGTAA

Structures

Toxin

• AlphaFold DB: H1G8D6

Antitoxin

• AlphaFold DB: H1G8D5