Detailed information of TA system TA242782

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 56823..57397
• Replicon: plasmid pl_URK_3a
• Accession: NZ_CP096130
• Organism: Leptospira interrogans strain KR40

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MY479_RS18150
• Protein ID: WP_025177725.1
• Coordinates: 57053..57397 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: M6U553
• Locus tag: MY479_RS18145
• Protein ID: WP_000443825.1
• Coordinates: 56823..57059 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MY479_RS18115 (MY479_17915)	52215..53084	+	870	WP_000041747.1	Rpn family recombination-promoting nuclease/putative transposase	-
MY479_RS18120 (MY479_17920)	53452..53871	-	420	WP_000921772.1	putative toxin-antitoxin system toxin component, PIN family	-
MY479_RS18125 (MY479_17925)	53871..54116	-	246	WP_001077269.1	ribbon-helix-helix domain-containing protein	-
MY479_RS18135 (MY479_17935)	55794..56006	+	213	WP_000447737.1	hypothetical protein	-
MY479_RS18140 (MY479_17940)	56055..56471	+	417	WP_000402981.1	hypothetical protein	-
MY479_RS18145 (MY479_17945)	56823..57059	+	237	WP_000443825.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
MY479_RS18150 (MY479_17950)	57053..57397	+	345	WP_025177725.1	endoribonuclease MazF	Toxin
MY479_RS18160 (MY479_17960)	58656..58967	-	312	WP_002188299.1	hypothetical protein	-
MY479_RS18165 (MY479_17965)	60071..61198	+	1128	Protein_45	IS3 family transposase	-
MY479_RS18170 (MY479_17970)	61221..61754	-	534	WP_002188141.1	transferase hexapeptide repeat protein	-
MY479_RS18175 (MY479_17975)	62079..62249	+	171	WP_002188134.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..85444	85444
	-	inside	IScluster/Tn	-	-	54344..61198	6854

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (11-111)

Antitoxin

• Antitoxin PemI-like (1-78)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13034.29 Da
• Isoelectric Point: 9.8852

>T242782 WP_025177725.1 NZ_CP096130:57053-57397 [Leptospira interrogans]
MVKYRNYTPEKGDIVWLNFTPQAGHEQKGRRPALVLSPKEYNSKTGLAIFCPITSKIKGYPFEVLIKSKKIDGVILSDQV
KNLDWTIREAEFIESINKVSLKEVLDNIKLLILT

Nucleotide

• Length: 345 bp

>T242782 NZ_OW967388:c298676-298374 [Enterobacter cloacae]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTGGGCCCGGGCTACAGAGTCTATTTTAAGGACCAGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8728.95 Da
• Isoelectric Point: 5.6663

>AT242782 WP_000443825.1 NZ_CP096130:56823-57059 [Leptospira interrogans]
MESIIQKWGNSLGIRIPKAMATELELNDGSHVELQYEGDKIVIYPMKKASLEDKLSKITKQNLHSEISTGNSIGNEAW

Nucleotide

• Length: 237 bp

>AT242782 NZ_OW967388:c298367-298080 [Enterobacter cloacae]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGGTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCAGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A0F6HC33