Detailed information of TA system TA242781

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 3263436..3264064
• Replicon: chromosome
• Accession: NZ_CP096129
• Organism: Leptospira interrogans strain KR40

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q8F7E4
• Locus tag: MY479_RS13810
• Protein ID: WP_000281042.1
• Coordinates: 3263666..3264064 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q8F7E3
• Locus tag: MY479_RS13805
• Protein ID: WP_001192482.1
• Coordinates: 3263436..3263666 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MY479_RS13770	3259258..3259392	+	135	WP_002188572.1	hypothetical protein	-
MY479_RS13775 (MY479_15285)	3259429..3259788	+	360	WP_002188605.1	tyrosine-type recombinase/integrase	-
MY479_RS13780 (MY479_15290)	3259820..3260842	+	1023	WP_000288518.1	hypothetical protein	-
MY479_RS13785 (MY479_15295)	3260897..3261424	+	528	WP_002188620.1	hypothetical protein	-
MY479_RS13790 (MY479_15300)	3261703..3262038	+	336	WP_000669388.1	type II toxin-antitoxin system RelE/ParE family toxin	-
MY479_RS13795 (MY479_15305)	3262035..3262334	+	300	WP_000769809.1	helix-turn-helix transcriptional regulator	-
MY479_RS13800 (MY479_15310)	3262455..3263084	+	630	WP_000639168.1	hypothetical protein	-
MY479_RS13805 (MY479_15315)	3263436..3263666	+	231	WP_001192482.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
MY479_RS13810 (MY479_15320)	3263666..3264064	+	399	WP_000281042.1	type II toxin-antitoxin system VapC family toxin	Toxin
MY479_RS13815 (MY479_15325)	3264162..3264461	+	300	Protein_2730	hypothetical protein	-
MY479_RS13820 (MY479_15330)	3264483..3264710	+	228	WP_000130601.1	hypothetical protein	-
MY479_RS13825 (MY479_15335)	3264852..3265928	+	1077	WP_001170569.1	hypothetical protein	-
MY479_RS13830 (MY479_15340)	3265929..3266387	+	459	WP_001239006.1	GNAT family N-acetyltransferase	-
MY479_RS13835 (MY479_15345)	3266604..3267466	-	863	Protein_2734	hypothetical protein	-
MY479_RS13840 (MY479_15350)	3267977..3268744	-	768	WP_000636953.1	DUF1554 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15139.76 Da
• Isoelectric Point: 9.6893

>T242781 WP_000281042.1 NZ_CP096129:3263666-3264064 [Leptospira interrogans]
MYLLDTNICIFLIKKKNATLLENLKKKLNKDLFVSSLTVAELEFGIQKSEFKEKNKVALIEFLTIFNILSFSDKDAESYG
IIRADLERKGNVIGSIDMLLAAQAIANNYIFVTNNTKEFKRIKALKIENWTQ

Nucleotide

• Length: 399 bp

>T242781 NZ_OW967388:c298367-298080 [Enterobacter cloacae]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGGTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCAGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8771.05 Da
• Isoelectric Point: 8.6588

>AT242781 WP_001192482.1 NZ_CP096129:3263436-3263666 [Leptospira interrogans]
MQTAKLFINGRSQAVRLPKEFQFTGDDVLIQKVGEAVILVPKNKAWNVFLEGLNGFSDDFFKEEREQPKSDKREKL

Nucleotide

• Length: 231 bp

>AT242781 NZ_OW967388:c298676-298374 [Enterobacter cloacae]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTGGGCCCGGGCTACAGAGTCTATTTTAAGGACCAGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Structures

Toxin

• AlphaFold DB: A0A0F6HAN6

Antitoxin

• AlphaFold DB: A0A0F6HAF7