242780

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpIK/PemK(toxin)
Location 1023308..1023884 Replicon chromosome
Accession NZ_CP096129
Organism Leptospira interrogans strain KR40

Toxin (Protein)


Gene name chpK Uniprot ID Q93MT8
Locus tag MY479_RS04500 Protein ID WP_000617906.1
Coordinates 1023543..1023884 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name chpI Uniprot ID Q93MT9
Locus tag MY479_RS04495 Protein ID WP_000844758.1
Coordinates 1023308..1023556 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MY479_RS04480 (MY479_06155) 1018959..1020941 + 1983 WP_000836894.1 GAF domain-containing protein -
MY479_RS04485 (MY479_06160) 1021583..1022041 - 459 WP_000453267.1 hypothetical protein -
MY479_RS04490 (MY479_06165) 1022195..1022491 + 297 WP_000477061.1 helix-turn-helix transcriptional regulator -
MY479_RS04495 (MY479_06170) 1023308..1023556 + 249 WP_000844758.1 ribbon-helix-helix domain-containing protein Antitoxin
MY479_RS04500 (MY479_06175) 1023543..1023884 + 342 WP_000617906.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MY479_RS04505 (MY479_06180) 1024978..1025490 + 513 WP_000079224.1 VOC family protein -
MY479_RS04510 (MY479_06185) 1026174..1026767 + 594 WP_001226990.1 dihydrofolate reductase family protein -
MY479_RS04515 (MY479_06190) 1026839..1027726 + 888 WP_002188401.1 hypothetical protein -
MY479_RS04520 (MY479_06195) 1027844..1028590 + 747 WP_000617027.1 GNAT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(2-41)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12372.25 Da        Isoelectric Point: 6.3272

>T242780 WP_000617906.1 NZ_CP096129:1023543-1023884 [Leptospira interrogans]
MIRGEIWWVDLGIPFGSEPGFKRPVLIIQDDSFNQSNINTIVSIAITSNLNLSEAPGNVFISKKDSSLSKDSVINVSQIV
TLDKERFLNKAGKLKSNKLGEVEIGLKLVTGLD

Download         Length: 342 bp

>T242780 NZ_CP096129:1023543-1023884 [Leptospira interrogans]
ATGATTCGTGGTGAAATTTGGTGGGTAGATTTGGGAATTCCATTTGGAAGTGAGCCTGGATTTAAACGTCCAGTTCTTAT
AATTCAAGATGACTCTTTTAATCAAAGTAATATTAATACAATAGTTTCGATTGCAATTACATCGAATTTAAATCTATCTG
AAGCACCAGGTAATGTTTTCATTAGTAAAAAAGATTCGAGTTTATCAAAGGACTCGGTCATAAATGTCTCTCAAATTGTG
ACTTTAGATAAAGAAAGATTTCTTAACAAAGCGGGTAAACTTAAATCAAATAAATTAGGTGAAGTTGAAATTGGGCTTAA
GTTGGTTACAGGCTTAGATTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9380.77 Da        Isoelectric Point: 10.3815

>AT242780 WP_000844758.1 NZ_CP096129:1023308-1023556 [Leptospira interrogans]
MKTAISIPDELFRAAEKIAKKLGIPRSQLFAKALEEFIQSHSKESVTEKLNKIYSNKSKETRNNITDLSVESLRKSLKND
SW

Download         Length: 249 bp

>AT242780 NZ_CP096129:1023308-1023556 [Leptospira interrogans]
ATGAAGACGGCGATTTCAATTCCTGATGAGTTATTTAGGGCTGCCGAGAAAATAGCTAAAAAACTTGGGATTCCTCGAAG
CCAGTTATTTGCAAAAGCATTAGAAGAATTTATCCAATCTCATAGTAAAGAATCTGTAACTGAGAAATTAAATAAAATCT
ACAGTAATAAATCCAAAGAAACTAGAAATAATATTACTGACTTATCCGTAGAATCGCTTCGTAAGAGTTTAAAAAATGAT
TCGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MS74

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HD92

References