Detailed information of TA system TA242775

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 13251..13835
• Replicon: plasmid pJNE5-64k
• Accession: NZ_CP096122
• Organism: Acinetobacter portensis strain JNE5

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N8Q7V3
• Locus tag: MZO21_RS14500
• Protein ID: WP_000286964.1
• Coordinates: 13533..13835 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: MZO21_RS14495
• Protein ID: WP_000985610.1
• Coordinates: 13251..13532 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MZO21_RS14470 (MZO21_14465)	8621..9145	-	525	WP_248104093.1	hypothetical protein	-
MZO21_RS14475 (MZO21_14470)	9428..10359	-	932	Protein_10	IS5-like element ISAba12 family transposase	-
MZO21_RS15130	10601..11839	+	1239	Protein_11	mechanosensitive ion channel	-
MZO21_RS14490 (MZO21_14485)	11983..13124	-	1142	Protein_12	IS4 family transposase	-
MZO21_RS14495 (MZO21_14490)	13251..13532	-	282	WP_000985610.1	putative addiction module antidote protein	Antitoxin
MZO21_RS14500 (MZO21_14495)	13533..13835	-	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MZO21_RS14505 (MZO21_14500)	13958..15272	-	1315	Protein_15	ISNCY family transposase	-
MZO21_RS14510 (MZO21_14505)	15564..15767	+	204	WP_067731914.1	hypothetical protein	-
MZO21_RS14515 (MZO21_14510)	16033..18003	+	1971	WP_248103958.1	choline BCCT transporter BetT	-
MZO21_RS14520 (MZO21_14515)	18156..18284	-	129	Protein_18	IS3 family transposase	-
MZO21_RS14525 (MZO21_14520)	18405..18707	-	303	WP_092818272.1	XRE family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..64629	64629
	-	inside	IScluster/Tn	-	-	5719..15284	9565

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-90)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11573.07 Da
• Isoelectric Point: 4.6838

>T242775 WP_000286964.1 NZ_CP096122:c13835-13533 [Acinetobacter portensis]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T242775 NZ_OW967374:c1308544-1308170 [Klebsiella oxytoca]
ATGCAAATACATTCCCTGTTCCTTCAAAGGGCGGCGTCGTCACGTCTGTCGTCAGTCGAAATCTGGCAAAAATTACTGGA
GTATCTGCTGGAGCAACACTACGGCCTTACCCTCAACGACACACCGTTTGGCAACGACAGTGTGATCCAGAAACACATTG
ATGCGGGAATCTCACTGTATGACGCTGTGAATTTTATCGTGGAAAAGTACGACCTGGTGCGTACTGACCGTCACGGCTTC
AGCGTGAAAGAGCAGTCTCCTTTTATCGGCAGCCTTGATATTCTCCGAGCCCGGAAGGCTACCGGGCTGATGACCCGCAA
GGGATATAAAACCGTTACTGACATCACAGGTGGCAGATTCAGCGGAGGGAAATAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT242775 WP_000985610.1 NZ_CP096122:c13532-13251 [Acinetobacter portensis]
MAIKLRKWDSAEHLKTEEDMQAYLQACIDESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT242775 NZ_OW967374:c1308953-1308594 [Klebsiella oxytoca]
ATGAAGAAAGCAACCCGTGTAATCAACCATAACATCACGGAACCCTGGTGGGGCCTCAGACGCAACATCACGCCGTGTTT
TGGTGCACGGCTGGTACAGGAGGGTAACCACCTGCATTACCTTGCTGACCGCGCCAGCATTGCCGGGACATTTAATGACG
CAGATTTACGCCATCTGGACCAGGTATTTCCGGTACTGATGAAGCAGATGGAGCTTATGCTGGCCAGCAGCGAACTCACG
CCCCGCATTCAGCGCTGCATCACGCTCCACGTAAAAGGGCTGATATGTGAGGCCGATACACTCGGCTCCTGCGGTTATTT
GTACATCGTAATTTATCCGGCGTCTGTAACAACCGAATAA

Structures

Toxin

• AlphaFold DB: A0A0A8TV94