Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 89575..90103 Replicon plasmid pJNE5-X3_NDM-1
Accession NZ_CP096121
Organism Acinetobacter portensis strain JNE5

Toxin (Protein)


Gene name relE Uniprot ID N9N871
Locus tag MZO21_RS13255 Protein ID WP_000221358.1
Coordinates 89575..89868 (-) Length 98 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID N9NWT9
Locus tag MZO21_RS13260 Protein ID WP_000246755.1
Coordinates 89858..90103 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MZO21_RS13240 (MZO21_13235) 86802..87965 - 1164 WP_032073150.1 efflux RND transporter periplasmic adaptor subunit -
MZO21_RS13245 (MZO21_13240) 88392..88595 + 204 Protein_95 LysE family translocator -
MZO21_RS13250 (MZO21_13245) 88659..89363 + 705 WP_001067858.1 IS6-like element IS26 family transposase -
MZO21_RS13255 (MZO21_13250) 89575..89868 - 294 WP_000221358.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MZO21_RS13260 (MZO21_13255) 89858..90103 - 246 WP_000246755.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
MZO21_RS13265 (MZO21_13260) 90300..91649 - 1350 WP_001060713.1 chromate efflux transporter -
MZO21_RS13270 (MZO21_13265) 91646..92572 - 927 WP_026055779.1 chromate resistance protein -
MZO21_RS13275 (MZO21_13270) 92687..93253 - 567 WP_000678311.1 recombinase family protein -
MZO21_RS13280 (MZO21_13275) 93985..94998 + 1014 WP_005006358.1 nucleotidyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCARB-5 / aac(3)-IId / mph(E) / msr(E) / aph(3'')-Ib / tet(X3) / blaNDM-1 / sul2 / lnu(G) / aph(3')-VIa - 1..333311 333311
- flank IS/Tn - - 88659..89363 704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(8-84)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-72)


Sequences

Toxin        


Download         Length: 98 a.a.        Molecular weight: 11292.52 Da        Isoelectric Point: 10.4753

>T242774 WP_000221358.1 NZ_CP096121:c89868-89575 [Acinetobacter portensis]
MTYKLLRHKDFTAEWEKLPVAIRDQFKKKLAKVIEQPHIPKNMLRGDLAGCYKIKLLKAGVRLVYQVKDDQVVILLITVG
KRADSIVYDEAKKRIKD

Download         Length: 294 bp

>T242774 NZ_OW967374:873322-873732 [Klebsiella oxytoca]
GTGGTCCTGTGGCAATCTGATTTACGCATCTCGTGGCGAGCGCAATGGTTCTCACTGTTGATGCACGGCGTGGTGGCGGC
GCTGGTGCTGGTGTTGCCCTGGCCGCTGAGCTATACCCCGCTGTGGCTGATACTGCTGTCGCTGGTGGTGTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGACAGGGGGAAATAAAGCTGCTGATTGACTCTCGCCTGCGCTGGCAAAAAGCG
GAGTGGGATATCGTCGGCACCCCGTGGGTAATCAATAGCGGAATGCTGCTGCGCCTGCGAAATACGGAAAACCAACGCAC
TCAGCATCTATGGGTGGCGGCGGATAGCATGGACGCGGGCGAGTGGCGCGATCTGCGTCGGCTGGTGTTACAGAAACCGA
CGCAAGATTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9249.64 Da        Isoelectric Point: 4.3142

>AT242774 WP_000246755.1 NZ_CP096121:c90103-89858 [Acinetobacter portensis]
MATVNFRIDEALKEKSYSILKEQGIAPTDFFTSILEYVATTGKLPVKKALLSEEDEELLALVRKRINDPKEMFEEVTLDD
L

Download         Length: 246 bp

>AT242774 NZ_OW967374:873075-873341 [Klebsiella oxytoca]
ATGGATATTAACAATAAAGCTCGCATCCACTGGGCATGCCGCCGGGGAATGCGTGAACTCGATATCTCCATCATGCCGTT
TTTTGAGCATGAGTACGATACGCTCAGTGATGACGATAAGCGGTTGTTCATCCGCCTGCTGGAAAATGACGATCCGGACC
TGTTCAACTGGTTGATGAACCATGGCGAACCGGCTGATGCAGAACTGCAGCGGATGGTAAAATTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6A1RKP4

Antitoxin

Source ID Structure
AlphaFold DB A0A6A1RJ80

References