Detailed information of TA system TA242773

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 78116..78700
• Replicon: plasmid pJNE5-X3_NDM-1
• Accession: NZ_CP096121
• Organism: Acinetobacter portensis strain JNE5

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N8Q7V3
• Locus tag: MZO21_RS13210
• Protein ID: WP_000286964.1
• Coordinates: 78398..78700 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: MZO21_RS13205
• Protein ID: WP_000985609.1
• Coordinates: 78116..78397 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MZO21_RS13175 (MZO21_13170)	73245..73580	+	336	WP_004780063.1	IS66 family insertion sequence element accessory protein TnpB	-
MZO21_RS13180 (MZO21_13175)	73655..75259	+	1605	WP_248129700.1	IS66 family transposase	-
MZO21_RS13185 (MZO21_13180)	75405..75677	-	273	WP_000369781.1	NadS family protein	-
MZO21_RS13190 (MZO21_13185)	75670..75990	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
MZO21_RS13195 (MZO21_13190)	76114..77439	-	1326	WP_139850444.1	ISNCY family transposase	-
MZO21_RS13200 (MZO21_13195)	77617..78036	+	420	WP_248104014.1	SMI1/KNR4 family protein	-
MZO21_RS13205 (MZO21_13200)	78116..78397	-	282	WP_000985609.1	putative addiction module antidote protein	Antitoxin
MZO21_RS13210 (MZO21_13205)	78398..78700	-	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MZO21_RS13220 (MZO21_13215)	80145..80873	+	729	WP_032073130.1	efflux system response regulator transcription factor AdeR	-
MZO21_RS13225 (MZO21_13220)	80863..81969	+	1107	WP_032073131.1	two-component sensor histidine kinase AdeS	-
MZO21_RS13230 (MZO21_13225)	82073..83587	-	1515	WP_032073148.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCARB-5 / aac(3)-IId / mph(E) / msr(E) / aph(3'')-Ib / tet(X3) / blaNDM-1 / sul2 / lnu(G) / aph(3')-VIa	-	1..333311	333311
	-	inside	IScluster/Tn	aac(3)-IId / mph(E) / msr(E)	-	42971..79872	36901

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-90)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11573.07 Da
• Isoelectric Point: 4.6838

>T242773 WP_000286964.1 NZ_CP096121:c78700-78398 [Acinetobacter portensis]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T242773 NZ_OW967374:c61391-61083 [Klebsiella oxytoca]
ATGCAGTATTACGTTTATAAGAATACAGGTCGAATTGCTGCGTATCCTTACCTCCTTGATGTACAGAGTGACATTATCGG
CGAGCGCAATACTCGGGTTGTGATTCCACTGTTTCCACTGAAAAACTATAAAGGTCCACGCGCCGATCGTTTAACACCGC
TTGTCACCGTAGAAGGGGAAGAATACGTCGTGATGACTCACGAACTCGCCAGCATCCCACATCGTGTTCTGGGTGAAGAG
GTTTGTAATCTGAATCACCAGCGCGAAGTCGTTAAAGCTTCAATGGATTTTCTTTTCGACGGGATCTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT242773 WP_000985609.1 NZ_CP096121:c78397-78116 [Acinetobacter portensis]
MAIKLRKWDSAEHLKTEDDMQAYLQACIEESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT242773 NZ_OW967374:c61630-61394 [Klebsiella oxytoca]
ATGCCAGCCGCAAAACGCAATAAGCAGAACGTCAGCGTGACACTGGACCCTGGCCTGGTCAAACAGGCTCGTGAGGTCGG
CATCAATCTGTCTCTAACGTTGACTAACGCTCTAAAAAGCGAAATCCACAGCCTCGAAATAGCCCGCTGGCAAAAAGAAA
ATGCCCTGGCAATCGACTACCTCAATAATTTAACTGAGGAAAATGGACTTCTCTCTGATGAATACAGGACGTTCTAA

Structures

Toxin

• AlphaFold DB: A0A0A8TV94