Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | BrnTA/BrnT-BrnA |
| Location | 59980..60568 | Replicon | plasmid pJNE5-X3_NDM-1 |
| Accession | NZ_CP096121 | ||
| Organism | Acinetobacter portensis strain JNE5 | ||
Toxin (Protein)
| Gene name | brnT | Uniprot ID | N8SM64 |
| Locus tag | MZO21_RS13100 | Protein ID | WP_000438825.1 |
| Coordinates | 60281..60568 (-) | Length | 96 a.a. |
Antitoxin (Protein)
| Gene name | brnA | Uniprot ID | D3JXQ4 |
| Locus tag | MZO21_RS13095 | Protein ID | WP_004728120.1 |
| Coordinates | 59980..60294 (-) | Length | 105 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MZO21_RS13065 (MZO21_13060) | 55967..56302 | - | 336 | WP_000618090.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| MZO21_RS13070 (MZO21_13065) | 56299..56727 | - | 429 | WP_000364662.1 | transposase | - |
| MZO21_RS13075 (MZO21_13070) | 56932..57162 | + | 231 | WP_248104292.1 | MerR family transcriptional regulator | - |
| MZO21_RS13080 (MZO21_13075) | 57216..57548 | - | 333 | Protein_62 | integrase core domain-containing protein | - |
| MZO21_RS13090 (MZO21_13085) | 58834..59636 | - | 803 | Protein_64 | IS3-like element ISAcsp5 family transposase | - |
| MZO21_RS13095 (MZO21_13090) | 59980..60294 | - | 315 | WP_004728120.1 | BrnA antitoxin family protein | Antitoxin |
| MZO21_RS13100 (MZO21_13095) | 60281..60568 | - | 288 | WP_000438825.1 | BrnT family toxin | Toxin |
| MZO21_RS13105 (MZO21_13100) | 61089..62218 | + | 1130 | WP_089024817.1 | IS3 family transposase | - |
| MZO21_RS13110 (MZO21_13105) | 62246..62659 | - | 414 | WP_248104159.1 | hypothetical protein | - |
| MZO21_RS13115 (MZO21_13110) | 62934..63245 | + | 312 | WP_038350243.1 | BrnT family toxin | - |
| MZO21_RS13120 (MZO21_13115) | 63208..63522 | + | 315 | WP_038350244.1 | BrnA antitoxin family protein | - |
| MZO21_RS13125 (MZO21_13120) | 63739..65019 | - | 1281 | WP_248104157.1 | cation diffusion facilitator family transporter | - |
| MZO21_RS13130 (MZO21_13125) | 65109..65501 | + | 393 | WP_000550240.1 | Cd(II)/Pb(II)-responsive transcriptional regulator | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | blaCARB-5 / aac(3)-IId / mph(E) / msr(E) / aph(3'')-Ib / tet(X3) / blaNDM-1 / sul2 / lnu(G) / aph(3')-VIa | - | 1..333311 | 333311 | |
| - | inside | IScluster/Tn | aac(3)-IId / mph(E) / msr(E) | - | 42971..79872 | 36901 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 96 a.a. Molecular weight: 11304.70 Da Isoelectric Point: 5.6205
>T242771 WP_000438825.1 NZ_CP096121:c60568-60281 [Acinetobacter portensis]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG
Download Length: 288 bp
>T242771 NZ_OW967265:c58286-57870 [Citrobacter freundii]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTACGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCTGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCGGCCAGTGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGATGCTGG
AAGACTGGGTAAAATAA
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTACGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCTGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCGGCCAGTGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGATGCTGG
AAGACTGGGTAAAATAA
Antitoxin
Download Length: 105 a.a. Molecular weight: 12200.86 Da Isoelectric Point: 8.4628
>AT242771 WP_004728120.1 NZ_CP096121:c60294-59980 [Acinetobacter portensis]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ
Download Length: 315 bp
>AT242771 NZ_OW967265:c58513-58283 [Citrobacter freundii]
ATGCGCACAGTTTCTATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCATTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA
ATGCGCACAGTTTCTATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCATTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0A8TVQ4 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0A8TRI9 |