Detailed information of TA system TA242766

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4700536..4701152
• Replicon: chromosome
• Accession: NZ_CP096118
• Organism: Salmonella enterica subsp. enterica serovar Indiana strain 22

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q8Z2U3
• Locus tag: MZK41_RS22880
• Protein ID: WP_000238494.1
• Coordinates: 4700536..4700910 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8E9YHE9
• Locus tag: MZK41_RS22885
• Protein ID: WP_001523745.1
• Coordinates: 4700910..4701152 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MZK41_RS22865 (4698038)	4698038..4698940	+	903	WP_051129234.1	formate dehydrogenase subunit beta	-
MZK41_RS22870 (4698937)	4698937..4699572	+	636	WP_000829028.1	formate dehydrogenase cytochrome b556 subunit	-
MZK41_RS22875 (4699569)	4699569..4700498	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
MZK41_RS22880 (4700536)	4700536..4700910	-	375	WP_000238494.1	type II toxin-antitoxin system VapC family toxin	Toxin
MZK41_RS22885 (4700910)	4700910..4701152	-	243	WP_001523745.1	CopG family transcriptional regulator	Antitoxin
MZK41_RS22890 (4701357)	4701357..4702286	+	930	WP_001162861.1	alpha/beta hydrolase	-
MZK41_RS22895 (4702372)	4702372..4702683	+	312	WP_000558165.1	type II toxin-antitoxin system HigB family toxin	-
MZK41_RS22900 (4702680)	4702680..4703126	+	447	WP_001259009.1	type II toxin-antitoxin system HigA family antitoxin	-
MZK41_RS22905 (4703141)	4703141..4704082	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
MZK41_RS22910 (4704127)	4704127..4704564	-	438	WP_000560969.1	D-aminoacyl-tRNA deacylase	-
MZK41_RS22915 (4704561)	4704561..4705433	-	873	WP_000921423.1	virulence factor BrkB family protein	-
MZK41_RS22920 (4705427)	4705427..4706026	-	600	WP_023227076.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13908.18 Da
• Isoelectric Point: 7.3567

>T242766 WP_000238494.1 NZ_CP096118:c4700910-4700536 [Salmonella enterica subsp. enterica serovar Indiana]
MVKGSALFDTNILIDLFSGRIEAKHALEAYPPQNAISLITWMEVMVGAKKYHQENRTRIALSAFNIIGVTQEIAERSVIV
RQEYGMKLPDAIILATAQVHRCELVTRNTKDFADIPGVITPYHL

Nucleotide

• Length: 375 bp

>T242766 NZ_OW967263:c4511106-4510822 [Citrobacter freundii]
ATGACTTATGAACTGGAATTCGACCCGAGAGCCTTAAAAGAGTGGCATAAACTGGGTGATACGGTGAAGGCTCAGTTTAA
GAAGAAGTTGGCCGACGTGTTGTTGAACCCCAGAATTGACTCTGCCCGTTTAAACGGTCTTCCAGATTGCTATAAAATTA
AGCTTAAATCGTCCGGCTATCGTCTGGTGTACCAGGTTCGGGATGAGGTTGTGACCGTGTTTGTTGTCGCGGTCGGCAAG
CGACAACATTCGGCCGTTTATCTCGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9437.77 Da
• Isoelectric Point: 4.4463

>AT242766 WP_001523745.1 NZ_CP096118:c4701152-4700910 [Salmonella enterica subsp. enterica serovar Indiana]
MMAGMDMGRILLDLSDDVIKRLDDLKVQRNLPRAELLREAVEQYLERQDRAETTISKALGLWQGCEEDGVEYQRKLREEW

Nucleotide

• Length: 243 bp

>AT242766 NZ_OW967263:c4511338-4511096 [Citrobacter freundii]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCTGTGCGATTACTGTTTCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACCGTTAGCGACA
GCGAAGATACACTGATTCAGACAGTCCGGGAGAGGCTGGCCAGCCCGCAAAAAGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• AlphaFold DB: A0A5I6CND8