Detailed information of TA system TA242737

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 82916..84024
• Replicon: plasmid pW142
• Accession: NZ_CP096052
• Organism: Enterococcus faecium strain AKSZ-142

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: A0A3S8RBH6
• Locus tag: MZO31_RS00450
• Protein ID: WP_071661597.1
• Coordinates: 82916..83785 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: MZO31_RS00455
• Protein ID: WP_000205227.1
• Coordinates: 83800..84024 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MZO31_RS00420 (MZO31_00420)	78701..79243	-	543	WP_000627290.1	streptothricin N-acetyltransferase Sat4	-
MZO31_RS00425 (MZO31_00425)	79240..80037	-	798	Protein_84	aminoglycoside 6-adenylyltransferase	-
MZO31_RS00430 (MZO31_00430)	80207..81646	-	1440	WP_001028144.1	aminoglycoside O-phosphotransferase APH(2'')-Ia	-
MZO31_RS00435 (MZO31_00435)	81647..82039	-	393	WP_000393259.1	GNAT family N-acetyltransferase	-
MZO31_RS00440 (MZO31_00440)	82058..82168	-	111	Protein_87	aminoglycoside 6-adenylyltransferase	-
MZO31_RS00445 (MZO31_00445)	82201..82935	-	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
MZO31_RS00450 (MZO31_00450)	82916..83785	-	870	WP_071661597.1	nucleotidyltransferase domain-containing protein	Toxin
MZO31_RS00455 (MZO31_00455)	83800..84024	-	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
MZO31_RS00460 (MZO31_00460)	84167..84301	-	135	Protein_91	site-specific recombinase	-
MZO31_RS00465 (MZO31_00465)	84442..85743	+	1302	Protein_92	ISL3 family transposase	-
MZO31_RS00470 (MZO31_00470)	86052..86855	-	804	WP_002294514.1	lincosamide nucleotidyltransferase Lnu(B)	-
MZO31_RS00475 (MZO31_00475)	86909..88393	-	1485	WP_002294513.1	ABC-F type ribosomal protection protein Lsa(E)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32902.55 Da
• Isoelectric Point: 4.9770

>T242737 WP_071661597.1 NZ_CP096052:c83785-82916 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEHKPEKYTE
KVNHIFEVLGISLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T242737 NZ_OW967003:4396343-4396678 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT242737 WP_000205227.1 NZ_CP096052:c84024-83800 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT242737 NZ_OW967003:4396095-4396343 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCTCCTGCAGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGAAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A3S8RBH6