Detailed information of TA system TA242736

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 116929..118037
• Replicon: plasmid pW47
• Accession: NZ_CP096051
• Organism: Enterococcus faecium strain AKSZ-47

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: A0A3S8RBH6
• Locus tag: MZO30_RS00610
• Protein ID: WP_071661597.1
• Coordinates: 116929..117798 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: MZO30_RS00615
• Protein ID: WP_000205227.1
• Coordinates: 117813..118037 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MZO30_RS00580 (MZO30_00580)	112714..113256	-	543	WP_000627290.1	streptothricin N-acetyltransferase Sat4	-
MZO30_RS00585 (MZO30_00585)	113253..114050	-	798	Protein_116	aminoglycoside 6-adenylyltransferase	-
MZO30_RS00590 (MZO30_00590)	114220..115659	-	1440	WP_001028144.1	aminoglycoside O-phosphotransferase APH(2'')-Ia	-
MZO30_RS00595 (MZO30_00595)	115660..116052	-	393	WP_204356999.1	GNAT family N-acetyltransferase	-
MZO30_RS00600 (MZO30_00600)	116071..116181	-	111	Protein_119	aminoglycoside 6-adenylyltransferase	-
MZO30_RS00605 (MZO30_00605)	116214..116948	-	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
MZO30_RS00610 (MZO30_00610)	116929..117798	-	870	WP_071661597.1	nucleotidyltransferase domain-containing protein	Toxin
MZO30_RS00615 (MZO30_00615)	117813..118037	-	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
MZO30_RS00620 (MZO30_00620)	118180..118314	-	135	Protein_123	site-specific recombinase	-
MZO30_RS00625 (MZO30_00625)	118455..119756	+	1302	Protein_124	ISL3 family transposase	-
MZO30_RS00630 (MZO30_00630)	120065..120868	-	804	WP_002294514.1	lincosamide nucleotidyltransferase Lnu(B)	-
MZO30_RS00635 (MZO30_00635)	120922..122406	-	1485	WP_002294513.1	ABC-F type ribosomal protection protein Lsa(E)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32902.55 Da
• Isoelectric Point: 4.9770

>T242736 WP_071661597.1 NZ_CP096051:c117798-116929 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEHKPEKYTE
KVNHIFEVLGISLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T242736 NZ_OW967003:4283373-4283780 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGAGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT242736 WP_000205227.1 NZ_CP096051:c118037-117813 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT242736 NZ_OW967003:4283126-4283392 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTCAACTGGCTGATGAATCATGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A3S8RBH6