Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 8762..9899 Replicon plasmid pW208
Accession NZ_CP096049
Organism Enterococcus faecalis strain AKSZ-208

Toxin (Protein)


Gene name zeta Uniprot ID -
Locus tag MZO28_RS00070 Protein ID WP_032495456.1
Coordinates 9036..9899 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag MZO28_RS00065 Protein ID WP_000301765.1
Coordinates 8762..9034 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MZO28_RS00035 (5762) 5762..6001 + 240 WP_002425012.1 peptide-binding protein -
MZO28_RS00040 (6065) 6065..6133 + 69 WP_011100846.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
MZO28_RS00045 (6258) 6258..6995 + 738 WP_248299236.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
MZO28_RS00050 (7165) 7165..7425 + 261 Protein_9 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
MZO28_RS00055 (7542) 7542..8438 + 897 WP_002387620.1 ParA family protein -
MZO28_RS00060 (8530) 8530..8745 + 216 WP_001835296.1 peptide-binding protein -
MZO28_RS00065 (8762) 8762..9034 + 273 WP_000301765.1 antitoxin Antitoxin
MZO28_RS00070 (9036) 9036..9899 + 864 WP_032495456.1 zeta toxin family protein Toxin
MZO28_RS00075 (10341) 10341..10658 + 318 WP_002300567.1 hypothetical protein -
MZO28_RS00080 (10677) 10677..11174 + 498 WP_248299237.1 molecular chaperone DnaJ -
MZO28_RS00085 (11208) 11208..11513 + 306 WP_141062716.1 hypothetical protein -
MZO28_RS00090 (11536) 11536..11793 - 258 WP_248299238.1 hypothetical protein -
MZO28_RS00095 (11835) 11835..12515 - 681 WP_218210647.1 IS6-like element IS1216 family transposase -
MZO28_RS00100 (12589) 12589..13044 + 456 Protein_19 transposase -
MZO28_RS00105 (13540) 13540..14139 + 600 WP_218210646.1 tyrosine-type recombinase/integrase -
MZO28_RS00110 (14187) 14187..14657 + 471 Protein_21 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) / fexA / optrA / aac(6')-aph(2'') - 1..56246 56246
- inside IScluster/Tn erm(B) - 1136..12912 11776


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32444.13 Da        Isoelectric Point: 6.9964

>T242731 WP_032495456.1 NZ_CP096049:9036-9899 [Enterococcus faecalis]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGIIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVASKEIQ
PTLERIEQKMILNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T242731 NZ_OW967003:c2672921-2672748 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGCGCATTCCTGGTCGGAATAACCTGGCCAATGAGTTTACCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT242731 WP_000301765.1 NZ_CP096049:8762-9034 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT242731 NZ_OW967003:c2673245-2672949 [Escherichia coli]
ATGGAAGGTAAAAATAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACCGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References