Detailed information of TA system TA242731

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 8762..9899
• Replicon: plasmid pW208
• Accession: NZ_CP096049
• Organism: Enterococcus faecalis strain AKSZ-208

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: MZO28_RS00070
• Protein ID: WP_032495456.1
• Coordinates: 9036..9899 (+)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: R2XCR7
• Locus tag: MZO28_RS00065
• Protein ID: WP_000301765.1
• Coordinates: 8762..9034 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MZO28_RS00035 (5762)	5762..6001	+	240	WP_002425012.1	peptide-binding protein	-
MZO28_RS00040 (6065)	6065..6133	+	69	WP_011100846.1	23S rRNA methyltransferase attenuator leader peptide ErmL	-
MZO28_RS00045 (6258)	6258..6995	+	738	WP_248299236.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
MZO28_RS00050 (7165)	7165..7425	+	261	Protein_9	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
MZO28_RS00055 (7542)	7542..8438	+	897	WP_002387620.1	ParA family protein	-
MZO28_RS00060 (8530)	8530..8745	+	216	WP_001835296.1	peptide-binding protein	-
MZO28_RS00065 (8762)	8762..9034	+	273	WP_000301765.1	antitoxin	Antitoxin
MZO28_RS00070 (9036)	9036..9899	+	864	WP_032495456.1	zeta toxin family protein	Toxin
MZO28_RS00075 (10341)	10341..10658	+	318	WP_002300567.1	hypothetical protein	-
MZO28_RS00080 (10677)	10677..11174	+	498	WP_248299237.1	molecular chaperone DnaJ	-
MZO28_RS00085 (11208)	11208..11513	+	306	WP_141062716.1	hypothetical protein	-
MZO28_RS00090 (11536)	11536..11793	-	258	WP_248299238.1	hypothetical protein	-
MZO28_RS00095 (11835)	11835..12515	-	681	WP_218210647.1	IS6-like element IS1216 family transposase	-
MZO28_RS00100 (12589)	12589..13044	+	456	Protein_19	transposase	-
MZO28_RS00105 (13540)	13540..14139	+	600	WP_218210646.1	tyrosine-type recombinase/integrase	-
MZO28_RS00110 (14187)	14187..14657	+	471	Protein_21	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	erm(B) / fexA / optrA / aac(6')-aph(2'')	-	1..56246	56246
	-	inside	IScluster/Tn	erm(B)	-	1136..12912	11776

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-214)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 32444.13 Da
• Isoelectric Point: 6.9964

>T242731 WP_032495456.1 NZ_CP096049:9036-9899 [Enterococcus faecalis]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGIIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVASKEIQ
PTLERIEQKMILNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Nucleotide

• Length: 864 bp

>T242731 NZ_OW967003:c2672921-2672748 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGCGCATTCCTGGTCGGAATAACCTGGCCAATGAGTTTACCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10721.25 Da
• Isoelectric Point: 6.4676

>AT242731 WP_000301765.1 NZ_CP096049:8762-9034 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Nucleotide

• Length: 273 bp

>AT242731 NZ_OW967003:c2673245-2672949 [Escherichia coli]
ATGGAAGGTAAAAATAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACCGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A829F0A3