Detailed information of TA system TA242725

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-ParD
• Location: 2574846..2575798
• Replicon: chromosome
• Accession: NZ_CP096035
• Organism: Rhodococcus pyridinivorans strain Y6

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: V9XD75
• Locus tag: MX572_RS12230
• Protein ID: WP_024101065.1
• Coordinates: 2574846..2575415 (-)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: V9X925
• Locus tag: MX572_RS12235
• Protein ID: WP_024101066.1
• Coordinates: 2575412..2575798 (-)
• Length: 129 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MX572_RS12230 (MX572_12225)	2574846..2575415	-	570	WP_024101065.1	zeta toxin family protein	Toxin
MX572_RS12235 (MX572_12230)	2575412..2575798	-	387	WP_024101066.1	hypothetical protein	Antitoxin
MX572_RS12240 (MX572_12235)	2576006..2577076	+	1071	WP_254582907.1	methyltransferase domain-containing protein	-
MX572_RS12245 (MX572_12240)	2577146..2577511	+	366	WP_064255686.1	HNH endonuclease	-
MX572_RS12250 (MX572_12245)	2577555..2578073	-	519	WP_252172755.1	hypothetical protein	-
MX572_RS12255 (MX572_12250)	2579188..2580120	-	933	WP_254582908.1	TAXI family TRAP transporter solute-binding subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (4-136)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (7-102)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 20524.65 Da
• Isoelectric Point: 10.6895

>T242725 WP_024101065.1 NZ_CP096035:c2575415-2574846 [Rhodococcus pyridinivorans]
MRRLDLIVGPNGSGKSTFVELSLAPLLPRSVFVNADVIARQRWPDRAEEMSYEAAQLAAAARDRLLRQGRSFIAETVFSH
PSKLELIDRAHEVGFTVVLHAMLVPEAMSVLRVQHRVASGGHAVPEDKIRARYRRLWPLVTEAIGRADQASVYDTASGTP
VVVARFAGGVPVGAVRWPGWAPAALTGLG

Nucleotide

• Length: 570 bp

>T242725 NZ_OW967003:c1845506-1845204 [Escherichia coli]
ATGGCTCAAAAGAATAATATAAGGAGTTTTCGTGATGCCTGGCTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTGTCTGGGAAATTACAGGAATATTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATATTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 129 a.a.
• Molecular weight: 13980.46 Da
• Isoelectric Point: 4.8349

>AT242725 WP_024101066.1 NZ_CP096035:c2575798-2575412 [Rhodococcus pyridinivorans]
MTSSTDKVTRFDAELVDSAIAEGGRQNRTGRQQLEYWARIGRAMTAHETASLHRVHEALAGTRELSELTAAEGRLFDAEI
DARLAGGLARTDYAEVLAARGVTTVVLDDEGRLVEQRPDGSRRVLDDA

Nucleotide

• Length: 387 bp

>AT242725 NZ_OW967003:c1845168-1844827 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTACTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAATAATCGTAAACTCACCACTG
AAATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Toxin

• AlphaFold DB: V9XD75

Antitoxin

• AlphaFold DB: V9X925