Detailed information of TA system TA024272

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 454381..455218
• Replicon: chromosome
• Accession: NC_013353
• Organism: Escherichia coli O103:H2 str. 12009

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: A0A0F5GET7
• Locus tag: ECO103_RS02110
• Protein ID: WP_000227783.1
• Coordinates: 454381..454923 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: ECO103_RS02115
• Protein ID: WP_001297137.1
• Coordinates: 454907..455218 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECO103_RS02090	449920..450831	-	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
ECO103_RS02095	450999..451490	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
ECO103_RS02100	451618..452982	-	1365	WP_001000978.1	MFS transporter	-
ECO103_RS02105	453390..454325	+	936	WP_001434881.1	sel1 repeat family protein	-
ECO103_RS02110	454381..454923	-	543	WP_000227783.1	GNAT family N-acetyltransferase	Toxin
ECO103_RS02115	454907..455218	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
ECO103_RS02120	455403..456293	-	891	WP_000971336.1	heme o synthase	-
ECO103_RS02125	456305..456634	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
ECO103_RS02130	456634..457248	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
ECO103_RS02135	457238..459229	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
ECO103_RS02140	459251..460198	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19776.96 Da
• Isoelectric Point: 8.0330

>T24272 WP_000227783.1 NC_013353:c454923-454381 [Escherichia coli O103:H2 str. 12009]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKQGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T24272 NC_013353:c454923-454381 [Escherichia coli O103:H2 str. 12009]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCAGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT24272 WP_001297137.1 NC_013353:c455218-454907 [Escherichia coli O103:H2 str. 12009]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT24272 NC_013353:c455218-454907 [Escherichia coli O103:H2 str. 12009]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A0F5GET7

Antitoxin

• AlphaFold DB: A0A0E0Y6B3