Detailed information of TA system TA242681

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3527862..3529082
• Replicon: chromosome
• Accession: NZ_CP095923
• Organism: Pseudomonas aeruginosa strain AR19438

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0H2Z8V4
• Locus tag: MX621_RS16560
• Protein ID: WP_003110488.1
• Coordinates: 3528504..3529082 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MX621_RS16555
• Protein ID: WP_003088112.1
• Coordinates: 3527862..3528416 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MX621_RS16530 (MX621_16530)	3523431..3523981	+	551	Protein_3279	XRE family transcriptional regulator	-
MX621_RS16535 (MX621_16535)	3523978..3524376	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
MX621_RS16540 (MX621_16540)	3524389..3524712	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
MX621_RS16545 (MX621_16545)	3525043..3525504	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
MX621_RS16550 (MX621_16550)	3525509..3527704	+	2196	WP_003110487.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
MX621_RS16555 (MX621_16555)	3527862..3528416	+	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
MX621_RS16560 (MX621_16560)	3528504..3529082	+	579	WP_003110488.1	HD domain-containing protein	Toxin
MX621_RS16565 (MX621_16565)	3529192..3530379	-	1188	WP_003110489.1	HlyD family efflux transporter periplasmic adaptor subunit	-
MX621_RS16570 (MX621_16570)	3530369..3532540	-	2172	WP_003110490.1	type I secretion system permease/ATPase	-
MX621_RS16575 (MX621_16575)	3532530..3533807	-	1278	WP_003110491.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (7-188)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21913.75 Da
• Isoelectric Point: 5.3655

>T242681 WP_003110488.1 NZ_CP095923:3528504-3529082 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDTPLRDRLLALWDEYERGETAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T242681 NZ_OW849377:1335893-1336291 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT242681 WP_003088112.1 NZ_CP095923:3527862-3528416 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT242681 NZ_OW849377:1335666-1335893 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Structures

Toxin

• AlphaFold DB: A0A0H2Z8V4