Detailed information of TA system TA242675

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3465100..3466321
• Replicon: chromosome
• Accession: NZ_CP095922
• Organism: Pseudomonas aeruginosa strain AR19583

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0C7CS07
• Locus tag: MX649_RS15800
• Protein ID: WP_003102740.1
• Coordinates: 3465743..3466321 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MX649_RS15795
• Protein ID: WP_003088112.1
• Coordinates: 3465100..3465654 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MX649_RS15770 (MX649_15770)	3460668..3461219	+	552	WP_003132721.1	XRE family transcriptional regulator	-
MX649_RS15775 (MX649_15775)	3461216..3461614	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
MX649_RS15780 (MX649_15780)	3461627..3461950	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
MX649_RS15785 (MX649_15785)	3462281..3462742	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
MX649_RS15790 (MX649_15790)	3462747..3464942	+	2196	WP_014603034.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
MX649_RS15795 (MX649_15795)	3465100..3465654	+	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
MX649_RS15800 (MX649_15800)	3465743..3466321	+	579	WP_003102740.1	HD domain-containing protein	Toxin
MX649_RS15805 (MX649_15805)	3466429..3467616	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
MX649_RS15810 (MX649_15810)	3467606..3469777	-	2172	WP_014603035.1	type I secretion system permease/ATPase	-
MX649_RS15815 (MX649_15815)	3469767..3471044	-	1278	WP_003102743.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21869.70 Da
• Isoelectric Point: 5.3655

>T242675 WP_003102740.1 NZ_CP095922:3465743-3466321 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T242675 NZ_OW849377:268890-269120 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT242675 WP_003088112.1 NZ_CP095922:3465100-3465654 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT242675 NZ_OW849377:269117-269476 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: A0A0C7CS07