Detailed information of TA system TA242674

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Acetyltransf_4(toxin)
• Location: 1820449..1821957
• Replicon: chromosome
• Accession: NZ_CP095922
• Organism: Pseudomonas aeruginosa strain AR19583

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: MX649_RS08460
• Protein ID: WP_012613851.1
• Coordinates: 1820449..1821036 (+)
• Length: 196 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9HYX2
• Locus tag: MX649_RS08465
• Protein ID: WP_003115566.1
• Coordinates: 1821103..1821957 (+)
• Length: 285 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MX649_RS08455 (MX649_08455)	1816704..1820183	-	3480	WP_003117605.1	PAS domain-containing hybrid sensor histidine kinase/response regulator	-
MX649_RS08460 (MX649_08460)	1820449..1821036	+	588	WP_012613851.1	GNAT family protein	Toxin
MX649_RS08465 (MX649_08465)	1821103..1821957	+	855	WP_003115566.1	AraC family transcriptional regulator	Antitoxin
MX649_RS08470 (MX649_08470)	1822054..1824219	+	2166	WP_003115567.1	TonB-dependent receptor family protein	-
MX649_RS08475 (MX649_08475)	1824394..1826268	+	1875	WP_014602678.1	MFS transporter	-
MX649_RS08480 (MX649_08480)	1826329..1826538	-	210	WP_003115568.1	cold-shock protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (20-152)

Antitoxin

• AraC-type arabinose-binding/dimerisation domain (19-151)

Sequences

Toxin

Protein

• Length: 196 a.a.
• Molecular weight: 21929.29 Da
• Isoelectric Point: 9.8676

>T242674 WP_012613851.1 NZ_CP095922:1820449-1821036 [Pseudomonas aeruginosa]
MSRRAPMFKPLPITLQRGALRLEPLVEADIPELVSLAEANREALQYMDGPTRPDWYRQSLAEQREGRALPLAVRLGVQLV
GTTRFAEFLPALPACEIGWTWLDQAQHGSGLNRMIKYLMLKHAFDNLRMVRVQLSTAASNLRAQGAIDKLGAQREGVLRN
HRRLAGGRLDDTFVYSITDHEWPQVKAVLEASFTG

Nucleotide

• Length: 588 bp

>T242674 NZ_OW849377:194224-194331 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin

Protein

• Length: 285 a.a.
• Molecular weight: 31933.23 Da
• Isoelectric Point: 7.3276

>AT242674 WP_003115566.1 NZ_CP095922:1821103-1821957 [Pseudomonas aeruginosa]
VPDETSGEHTHFWRAAELGGVELLHARYVEQRFAPHVHSSFALVIIEQGAQRFRHRGGEHLAPLGSMVLINPDEVHTGSK
AHDAGWLYRGYYPEMEQIDGILDELEIHHGGVPRFADSVIHDQPLARAFVHLHRLLAEHADSLETQTRWRETVLALFRRH
ARLPEAPKAGQEPLAVARARELLAARLLDPPSLEELAAAVNLSPFHFARVFRRATGMPPHAWLRQRRLEQARALLKSGRA
PLEVALELGFADQSHLGRQFKQAYGVAPGEYRSACARSSLTIYE

Nucleotide

• Length: 855 bp

>AT242674 NZ_OW849377:c194167-194112 [Escherichia coli]
TCAAGATTAGCCCCCGTTATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Structures

Antitoxin

• AlphaFold DB: Q9HYX2