Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 240044..240686 Replicon chromosome
Accession NZ_CP095874
Organism Bacillus sp. PGP15

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag MU858_RS01355 Protein ID WP_000635963.1
Coordinates 240336..240686 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag MU858_RS01350 Protein ID WP_000004570.1
Coordinates 240044..240331 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU858_RS01325 (MU858_01325) 235213..236175 + 963 WP_048529174.1 UV DNA damage repair endonuclease UvsE -
MU858_RS01330 (MU858_01330) 236338..236886 - 549 WP_098345068.1 rhomboid family intramembrane serine protease -
MU858_RS01335 (MU858_01335) 236979..237338 + 360 WP_048529178.1 holo-ACP synthase -
MU858_RS01340 (MU858_01340) 237495..238445 + 951 WP_002096914.1 outer membrane lipoprotein carrier protein LolA -
MU858_RS01345 (MU858_01345) 238564..239733 + 1170 WP_166689705.1 alanine racemase -
MU858_RS01350 (MU858_01350) 240044..240331 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
MU858_RS01355 (MU858_01355) 240336..240686 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
MU858_RS01360 (MU858_01360) 240755..242923 + 2169 WP_166689704.1 Tex family protein -
MU858_RS01365 (MU858_01365) 242982..243098 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
MU858_RS01370 (MU858_01370) 243294..243752 + 459 WP_048529183.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T242665 WP_000635963.1 NZ_CP095874:240336-240686 [Bacillus sp. PGP15]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T242665 NZ_OW849195:454014-454364 [Enterobacter cloacae]
ATGGTGAAGCGACCTTGTTTTGAACGAGGTGATATCGTTCTCGTCGGCTTCGATCCTGCAAATGGTCATGAACAAAAGGG
CGCAGGCAGGCCCGCTTTAGTGCTATCCGTTAGCGCGTTCAACCAGCTGGGAATGACGCTGGTTGCGCCTGTTACACAGG
GGGGAAATTTTGCCCGCTATGCGGGTTTCAGTGTGCCGCTGTCTTGTGAAGAAGGGAATATTCTGGGCGTCATTCTGGTA
AACCAAATCCGAATGATGGATTTGGGCGCGCGTCTGGCAAAACGAATAGGCGTGGCATCAGATGAAACCGTAGAAGATGC
GCTTTTAAGGCTACAGGCCATCCTGAGTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT242665 WP_000004570.1 NZ_CP095874:240044-240331 [Bacillus sp. PGP15]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT242665 NZ_OW849195:453769-454020 [Enterobacter cloacae]
ATGCGTATAACCATTAAGAAATGGGGAAACAGTGCCGGTATGGTCATTCCTGGCGTAGTAATGAAAGAACTTGGCCTAAA
ACCAGGTCAGAGTATGGAGGCTCAGGTGACAAATAATCAGTTAGTGCTTACACCGGTGAGTAAACAGTACTCGCTTGAAG
AGTTGTTGGCGCAGTGTGATATGTCAGCCCCGGAAATTAACGAACAGGACGTATGGGGCACGTCAGGTCCCATAGGTGAC
GAAGTATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References