Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | BrnTA/BrnT-BrnA |
| Location | 53648..54236 | Replicon | plasmid p11J360-1 |
| Accession | NZ_CP095868 | ||
| Organism | Acinetobacter nosocomialis strain 11J360 | ||
Toxin (Protein)
| Gene name | brnT | Uniprot ID | N8SM64 |
| Locus tag | MW889_RS18935 | Protein ID | WP_000438825.1 |
| Coordinates | 53949..54236 (-) | Length | 96 a.a. |
Antitoxin (Protein)
| Gene name | brnA | Uniprot ID | D3JXQ4 |
| Locus tag | MW889_RS18930 | Protein ID | WP_004728120.1 |
| Coordinates | 53648..53962 (-) | Length | 105 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MW889_RS18895 (MW889_18890) | 49785..50057 | - | 273 | WP_000369781.1 | NadS family protein | - |
| MW889_RS18900 (MW889_18895) | 50050..50370 | - | 321 | WP_000897307.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| MW889_RS18905 (MW889_18900) | 50510..50929 | + | 420 | WP_001180106.1 | SMI1/KNR4 family protein | - |
| MW889_RS18910 (MW889_18905) | 51009..51290 | - | 282 | WP_000985609.1 | putative addiction module antidote protein | - |
| MW889_RS18915 (MW889_18910) | 51291..51593 | - | 303 | WP_000286964.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| MW889_RS18920 (MW889_18915) | 51742..52125 | + | 384 | WP_257229894.1 | hypothetical protein | - |
| MW889_RS18925 (MW889_18920) | 52143..53276 | - | 1134 | WP_171265713.1 | IS3-like element ISAba14 family transposase | - |
| MW889_RS18930 (MW889_18925) | 53648..53962 | - | 315 | WP_004728120.1 | BrnA antitoxin family protein | Antitoxin |
| MW889_RS18935 (MW889_18930) | 53949..54236 | - | 288 | WP_000438825.1 | BrnT family toxin | Toxin |
| MW889_RS18940 (MW889_18935) | 54500..55834 | - | 1335 | WP_203080106.1 | ISNCY family transposase | - |
| MW889_RS18945 (MW889_18940) | 55971..56303 | - | 333 | WP_117008139.1 | MerR family DNA-binding protein | - |
| MW889_RS18950 (MW889_18945) | 56329..56712 | + | 384 | WP_001055589.1 | transposase | - |
| MW889_RS18955 (MW889_18950) | 56709..57044 | + | 336 | WP_000618091.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| MW889_RS18960 (MW889_18955) | 57119..58765 | + | 1647 | WP_001094417.1 | IS66-like element ISAba16 family transposase | - |
| MW889_RS18965 (MW889_18960) | 58796..58960 | + | 165 | Protein_69 | integrase core domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | floR / tet(M) / mph(E) / msr(E) / aac(3)-IId / aph(3')-VIa / sul2 / tet(X3) | - | 1..285025 | 285025 | |
| - | inside | IScluster/Tn | floR / tet(M) / mph(E) / msr(E) / aac(3)-IId | - | 37568..67377 | 29809 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 96 a.a. Molecular weight: 11304.70 Da Isoelectric Point: 5.6205
>T242662 WP_000438825.1 NZ_CP095868:c54236-53949 [Acinetobacter nosocomialis]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG
Download Length: 288 bp
>T242662 NZ_OW849099:c4279179-4278895 [Citrobacter freundii]
ATGACTTATGAACTGGAATTCGACCCGAGAGCCTTAAAAGAGTGGCATAAACTGGGTGATACGGTGAAGGCTCAGTTTAA
GAAGAAGTTGGCCGACGTGTTGTTGAACCCCAGAATTGACTCTGCCCGTTTAAACGGTCTTCCAGATTGCTATAAAATTA
AGCTTAAATCGTCCGGCTATCGTCTGCTGTACCAGGTTCGGGATGAGGTTGTGATCGTGTTTGTTGTCGCGGTCGGCAAG
CGAGAACATTCGGCCGTTTATCTCGATGCAAACAAACGGCTTTAG
ATGACTTATGAACTGGAATTCGACCCGAGAGCCTTAAAAGAGTGGCATAAACTGGGTGATACGGTGAAGGCTCAGTTTAA
GAAGAAGTTGGCCGACGTGTTGTTGAACCCCAGAATTGACTCTGCCCGTTTAAACGGTCTTCCAGATTGCTATAAAATTA
AGCTTAAATCGTCCGGCTATCGTCTGCTGTACCAGGTTCGGGATGAGGTTGTGATCGTGTTTGTTGTCGCGGTCGGCAAG
CGAGAACATTCGGCCGTTTATCTCGATGCAAACAAACGGCTTTAG
Antitoxin
Download Length: 105 a.a. Molecular weight: 12200.86 Da Isoelectric Point: 8.4628
>AT242662 WP_004728120.1 NZ_CP095868:c53962-53648 [Acinetobacter nosocomialis]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ
Download Length: 315 bp
>AT242662 NZ_OW849099:c4279411-4279169 [Citrobacter freundii]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCTGTGCGATTACTGTTTCAGTACGTTGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACCGTTAGCGACA
GCGAAGATACACTGATTCAGACAGTCCGGGAGAGGCTGGCCAGCCCGCAAAAAGGAATCAAGGTTAGTCTGGATGACTTA
TGA
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCTGTGCGATTACTGTTTCAGTACGTTGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACCGTTAGCGACA
GCGAAGATACACTGATTCAGACAGTCCGGGAGAGGCTGGCCAGCCCGCAAAAAGGAATCAAGGTTAGTCTGGATGACTTA
TGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0A8TVQ4 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0A8TRI9 |