Detailed information of TA system TA242661

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 51009..51593
• Replicon: plasmid p11J360-1
• Accession: NZ_CP095868
• Organism: Acinetobacter nosocomialis strain 11J360

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N8Q7V3
• Locus tag: MW889_RS18915
• Protein ID: WP_000286964.1
• Coordinates: 51291..51593 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: MW889_RS18910
• Protein ID: WP_000985609.1
• Coordinates: 51009..51290 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW889_RS18885 (MW889_18880)	46877..47761	-	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
MW889_RS18890 (MW889_18885)	47817..49292	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
MW889_RS18895 (MW889_18890)	49785..50057	-	273	WP_000369781.1	NadS family protein	-
MW889_RS18900 (MW889_18895)	50050..50370	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
MW889_RS18905 (MW889_18900)	50510..50929	+	420	WP_001180106.1	SMI1/KNR4 family protein	-
MW889_RS18910 (MW889_18905)	51009..51290	-	282	WP_000985609.1	putative addiction module antidote protein	Antitoxin
MW889_RS18915 (MW889_18910)	51291..51593	-	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MW889_RS18920 (MW889_18915)	51742..52125	+	384	WP_257229894.1	hypothetical protein	-
MW889_RS18925 (MW889_18920)	52143..53276	-	1134	WP_171265713.1	IS3-like element ISAba14 family transposase	-
MW889_RS18930 (MW889_18925)	53648..53962	-	315	WP_004728120.1	BrnA antitoxin family protein	-
MW889_RS18935 (MW889_18930)	53949..54236	-	288	WP_000438825.1	BrnT family toxin	-
MW889_RS18940 (MW889_18935)	54500..55834	-	1335	WP_203080106.1	ISNCY family transposase	-
MW889_RS18945 (MW889_18940)	55971..56303	-	333	WP_117008139.1	MerR family DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	floR / tet(M) / mph(E) / msr(E) / aac(3)-IId / aph(3')-VIa / sul2 / tet(X3)	-	1..285025	285025
	-	inside	IScluster/Tn	floR / tet(M) / mph(E) / msr(E) / aac(3)-IId	-	37568..67377	29809

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-90)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11573.07 Da
• Isoelectric Point: 4.6838

>T242661 WP_000286964.1 NZ_CP095868:c51593-51291 [Acinetobacter nosocomialis]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T242661 NZ_OW849099:3578491-3578709 [Citrobacter freundii]
ATGTCCGATAAACCATTAACTAAAACAGATTATTTGATGCGCCTGCGACGTTGCCAGACAATTGATACGCTAGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCATCGCCTTGCTGAAT
TGACCATGAATAAGCTTTACGACAAGATTCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT242661 WP_000985609.1 NZ_CP095868:c51290-51009 [Acinetobacter nosocomialis]
MAIKLRKWDSAEHLKTEDDMQAYLQACIEESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT242661 NZ_OW849099:3578089-3578463 [Citrobacter freundii]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACTCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAATGACCCTACCTCTGCAACCAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATCGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAGTCAGGTAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A0A8TV94