Detailed information of TA system TA242656

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 2091502..2092154
• Replicon: chromosome
• Accession: NZ_CP095864
• Organism: Gordonia sp. PP30

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MYK68_RS09580
• Protein ID: WP_247867763.1
• Coordinates: 2091502..2091921 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MYK68_RS09585
• Protein ID: WP_247867764.1
• Coordinates: 2091918..2092154 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYK68_RS09545 (MYK68_09545)	2087100..2088101	+	1002	WP_247867757.1	IS481 family transposase	-
MYK68_RS09550 (MYK68_09550)	2088098..2088442	-	345	WP_247867758.1	RNA polymerase-binding protein RbpA	-
MYK68_RS09555 (MYK68_09555)	2088614..2088895	+	282	WP_247867995.1	hypothetical protein	-
MYK68_RS09560 (MYK68_09560)	2088927..2089781	+	855	WP_247867759.1	adenylate/guanylate cyclase domain-containing protein	-
MYK68_RS09565 (MYK68_09565)	2089778..2090053	+	276	WP_247867760.1	hypothetical protein	-
MYK68_RS09570 (MYK68_09570)	2090106..2090348	-	243	WP_247867761.1	hypothetical protein	-
MYK68_RS09575 (MYK68_09575)	2090405..2091442	-	1038	WP_247867762.1	LLM class F420-dependent oxidoreductase	-
MYK68_RS09580 (MYK68_09580)	2091502..2091921	-	420	WP_247867763.1	type II toxin-antitoxin system VapC family toxin	Toxin
MYK68_RS09585 (MYK68_09585)	2091918..2092154	-	237	WP_247867764.1	Arc family DNA-binding protein	Antitoxin
MYK68_RS09590 (MYK68_09590)	2092206..2092571	-	366	WP_247867765.1	hypothetical protein	-
MYK68_RS09595 (MYK68_09595)	2092568..2093893	-	1326	WP_247867766.1	PepSY-associated TM helix domain-containing protein	-
MYK68_RS09600 (MYK68_09600)	2094022..2096256	-	2235	WP_247867767.1	catalase/peroxidase HPI	-
MYK68_RS09605 (MYK68_09605)	2096316..2096786	-	471	WP_247867768.1	Fur family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2087100..2088101	1001

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-128)

Antitoxin

• Arc-like DNA binding domain (15-42)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14949.93 Da
• Isoelectric Point: 4.1604

>T242656 WP_247867763.1 NZ_CP095864:c2091921-2091502 [Gordonia sp. PP30]
MIVLDTNVLSELFRPEPDRHVLEWLDDQDAADTATTAVTSAELLYGAARLPAGRRRTALAAAITAVLDEDLAGRILPFDS
VAAVHYAEIVTERERLGLPIGAADAQIAAICAAEDATLATRNIKDFQNIGIDLINPWTV

Nucleotide

• Length: 420 bp

>T242656 NZ_OW849099:260924-261292 [Citrobacter freundii]
ATGACGCTACAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTGACGCCTGGTGTCGCAGG
TATGCCTGACCCCGGTCGTGCGGAAGCGCTGATGTATCGGGTATTGAATCAAATCGAATATGAAGGTGTGACTGACGTGT
GGCTACTGGCGGCAATGCATTTGTTAGCCATTTCCCGTGGGCATATCTTTAATGATGGTAACAAGCGTACTGCGTTATTT
ATTACGTTGCTGTTTTTAAAACGTAATGGGATCGTATTATCTGCAAACCCTGATTTTGTCGCCATGACGGTTGAAGCGGC
AGCAGGACAACTCACGTTAGAACAAATTGCGCATCGTCTGCGCCATTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8647.67 Da
• Isoelectric Point: 6.7264

>AT242656 WP_247867764.1 NZ_CP095864:c2092154-2091918 [Gordonia sp. PP30]
MATLTIRGFDDDLHAKLRVRAAQHGRSMEAEVRAILEDDLTRSPKRSGLGSRIHARFAELGDFETSRATDAPRAADLE

Nucleotide

• Length: 237 bp

>AT242656 NZ_OW849099:260706-260927 [Citrobacter freundii]
ATGCGCACTATGAACTATAGTGAAGCTCGACAAAACCTGGCTGAAGTGCTGGAGAGCGCTGTCACTGGTGTGCCTGTTAC
CATTACGCGCCGTGGTCATAAACCTGCGGTAATCATCAGCGCGGAAGAGTTTGAACGCTATCAGGCCGCCAGACTTGATG
ATGAATTTGCCGACATAATGATGATTCATGGTAATGAAATCAGGGAGCTGGCGGATAAATGA