Detailed information of TA system TA242655

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 4859977..4860613
• Replicon: chromosome
• Accession: NZ_CP095860
• Organism: Bacillus sp. H8-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D5DWT4
• Locus tag: MT476_RS25100
• Protein ID: WP_013055004.1
• Coordinates: 4859977..4860327 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: D5DWT3
• Locus tag: MT476_RS25105
• Protein ID: WP_013055003.1
• Coordinates: 4860332..4860613 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MT476_RS25065 (MT476_25065)	4855561..4856355	-	795	WP_013055011.1	RNA polymerase sigma factor SigB	-
MT476_RS25070 (MT476_25070)	4856321..4856806	-	486	WP_025753516.1	anti-sigma B factor RsbW	-
MT476_RS25075 (MT476_25075)	4856803..4857135	-	333	WP_013055009.1	anti-sigma factor antagonist	-
MT476_RS25080 (MT476_25080)	4857195..4858205	-	1011	WP_013055008.1	PP2C family protein-serine/threonine phosphatase	-
MT476_RS25085 (MT476_25085)	4858219..4858620	-	402	WP_013055007.1	anti-sigma regulatory factor	-
MT476_RS25090 (MT476_25090)	4858624..4858980	-	357	WP_013055006.1	STAS domain-containing protein	-
MT476_RS25095 (MT476_25095)	4858983..4859819	-	837	WP_013055005.1	RsbT co-antagonist protein RsbRA	-
MT476_RS25100 (MT476_25100)	4859977..4860327	-	351	WP_013055004.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
MT476_RS25105 (MT476_25105)	4860332..4860613	-	282	WP_013055003.1	hypothetical protein	Antitoxin
MT476_RS25110 (MT476_25110)	4860814..4862004	-	1191	WP_176345086.1	alanine racemase	-
MT476_RS25115 (MT476_25115)	4862118..4863176	-	1059	WP_033580802.1	outer membrane lipoprotein carrier protein LolA	-
MT476_RS25120 (MT476_25120)	4863235..4863600	-	366	WP_028411881.1	holo-ACP synthase	-
MT476_RS25125 (MT476_25125)	4863665..4864267	+	603	WP_028411882.1	rhomboid family intramembrane serine protease	-
MT476_RS25130 (MT476_25130)	4864276..4865247	-	972	WP_028411883.1	UV DNA damage repair endonuclease UvsE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12991.96 Da
• Isoelectric Point: 4.8781

>T242655 WP_013055004.1 NZ_CP095860:c4860327-4859977 [Bacillus sp. H8-1]
MVVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVDEALQISVGLIDF

Nucleotide

• Length: 351 bp

>T242655 NZ_OW849099:235848-236123 [Citrobacter freundii]
ATGAAGGAAAAAGTGTTGTCTTTACGCAAAAAGCAAAAGAACACGCTGGATCAGCTTTTTAAAGCACCGACGCCGCAGGG
AATCAAGTGGTCGGAAATTGAGTCACTGATCAAGGCGTTAGGTGGCGAAATCAAAGAAGGACGAGGCTCGCGATGTAAAT
TTCTTTTAAATAAGAGCATTGCGAGTTTTCATAGACCACATCCTTCTCCGGATACAGATAAAGGCGCGGTCGAAAACGTA
CGTGACTGGTTAACAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10691.22 Da
• Isoelectric Point: 8.5339

>AT242655 WP_013055003.1 NZ_CP095860:c4860613-4860332 [Bacillus sp. H8-1]
VSEASATTEILVRLPKNLVSELDLMVKQENGNRSDFIHQATKLYLRERKKRHIRESMRRGYMEMAKINLNIASEAFLAEY
EADHTVDRLVSGG

Nucleotide

• Length: 282 bp

>AT242655 NZ_OW849099:236120-236479 [Citrobacter freundii]
ATGATCAAACCCAAAACGCCAAACTCAATGGAAATCGCCGGTCAACCTGCGGTGATTAATTATGTCCCTGAGCTTAATGC
GTTTCGCGGTAAGTTCCTGGGATTGTCAGGCTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAGGGGG
AGATCTCCTTACGAGAGTATCTTGATGATTGCTCTGCCGCAGGGATAGAACCTTACGCAAACCCGGAAAAGCTGAAGACG
TTTACTCTGCGCTATCCAGAGTCTTTCGGAGAACGTTTAAGTTTTGCGGCAGCGGAAGAACAGGTATCAGTCAATACATG
GATCATCGAAACGCTCAGCAAAAGTCTCAAACAGGCATAG

Structures

Antitoxin

• AlphaFold DB: A0A0B6AMB9