Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 40768..41411 Replicon plasmid pEC6563-3
Accession NZ_CP095859
Organism Escherichia coli strain EC6563

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag MYG04_RS25545 Protein ID WP_001034044.1
Coordinates 40995..41411 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag MYG04_RS25540 Protein ID WP_001261286.1
Coordinates 40768..40998 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MYG04_RS25525 (MYG04_25525) 35905..36135 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -
MYG04_RS25530 (MYG04_25530) 36132..36548 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
MYG04_RS25535 (MYG04_25535) 36593..40387 - 3795 WP_001144732.1 hypothetical protein -
MYG04_RS25540 (MYG04_25540) 40768..40998 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MYG04_RS25545 (MYG04_25545) 40995..41411 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
MYG04_RS25550 (MYG04_25550) 41486..43051 + 1566 WP_001128474.1 AAA family ATPase -
MYG04_RS25555 (MYG04_25555) 43036..44058 + 1023 WP_000361402.1 helicase UvrD -
MYG04_RS25565 (MYG04_25565) 45365..46279 + 915 WP_000949004.1 iron/manganese ABC transporter substrate-binding protein SitA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(B) / sitABCD iucA / iucB / iucC / iucD / iutA 1..63122 63122
- flank IS/Tn sitABCD - 44734..48814 4080


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T242654 WP_001034044.1 NZ_CP095859:40995-41411 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T242654 NZ_OW849099:30120-30482 [Citrobacter freundii]
ATGTGGGAAGTGGAAACAACGGATGCTTTCGATAAATGGTTTGACGTACAGACCGAAGCGCTCAAGGAAGATATGCTCGC
AGCAATGATGATTTTATCCGAGTATGGTCCTCAGTTAGGCAGGCCGTTTGCTGATACGGTAAATGCTTCTGCTTTTTCAA
ATATGAAAGAGTTGCGAGTACAGCATCAGGGTAGTCCGATTCGGGCGTTCTTCGCGTTTGATCCTTCTCGCCATGGCATC
GTGCTATGCGCTGGAGATAAAACAGGGTTAAACGAAAAGAAATTCTATAAAGAGATGATCAGGCTTGCGGATGCTGAGTA
CCGAAATCACCTAATAAGTAAGGAAAATTATGGCTACCCTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT242654 WP_001261286.1 NZ_CP095859:40768-40998 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT242654 NZ_OW849099:30469-30768 [Citrobacter freundii]
ATGGCTACCCTTAAAGAATTGATGGCGAAACAAAGCCCGGAAAGCCAGGAGCGTATTACCGAAAAAGTAGAAATACTACG
TCAGGCGGTTGCGTTAAATATGTTAAGGGAAGAACTGAATCTCTCTCAGGCTGAACTTGCCTCGGCAATGGGGGTTAAGC
AGCCAACAATCGCAAAAATGGAGCAGGCAGATAACGATCCTCGTCTGTCGACGCTCAAGCGTTATGTCACGGCACTGGGT
GGTGAACTTAGCATTGATGTTAAGCTACCTACAGGTAAAAGAGTCGCGTTCTATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References