Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 90427..92050 Replicon plasmid pEC6563-MCR
Accession NZ_CP095857
Organism Escherichia coli strain EC6563

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag MYG04_RS23945 Protein ID WP_000088046.1
Coordinates 90725..92050 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag MYG04_RS23940 Protein ID WP_024136327.1
Coordinates 90427..90720 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MYG04_RS23915 (MYG04_23915) 86695..87669 - 975 WP_012478345.1 IS30-like element ISApl1 family transposase -
MYG04_RS23920 (MYG04_23920) 88103..88384 - 282 WP_000077926.1 hypothetical protein -
MYG04_RS23925 (MYG04_23925) 88434..88625 - 192 WP_000398480.1 hypothetical protein -
MYG04_RS23930 (MYG04_23930) 88717..89058 - 342 WP_001151575.1 hypothetical protein -
MYG04_RS23935 (MYG04_23935) 89431..89823 + 393 WP_000341066.1 hypothetical protein -
MYG04_RS23940 (MYG04_23940) 90427..90720 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
MYG04_RS23945 (MYG04_23945) 90725..92050 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
MYG04_RS23950 (MYG04_23950) 92111..92317 + 207 WP_000134171.1 hypothetical protein -
MYG04_RS23955 (MYG04_23955) 92419..92829 + 411 WP_000985911.1 hypothetical protein -
MYG04_RS23960 (MYG04_23960) 92842..93657 + 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
MYG04_RS23965 (MYG04_23965) 93911..94336 + 426 WP_001043843.1 hypothetical protein -
MYG04_RS23970 (MYG04_23970) 94790..95025 + 236 Protein_91 type I restriction-modification system subunit M N-terminal domain-containing protein -
MYG04_RS23975 (MYG04_23975) 95080..95379 + 300 WP_001572440.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-1.1 / oqxB / oqxA / sul1 / dfrA12 / aph(3')-Ia / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / floR / sul2 / aph(4)-Ia / aac(3)-IVa / blaCTX-M-14 / fosA3 - 1..230727 230727
- flank IS/Tn mcr-1.1 - 84874..87618 2744


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T242651 WP_000088046.1 NZ_CP095857:90725-92050 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T242651 NZ_OW849083:c71969-71553 [Citrobacter freundii]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTACGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCTGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCGGCCAGTGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGATGCTGG
AAGACTGGGTAAAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT242651 WP_024136327.1 NZ_CP095857:90427-90720 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT242651 NZ_OW849083:c72196-71966 [Citrobacter freundii]
ATGCGCACAGTTTCTATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCATTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References