Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4333006..4333624 Replicon chromosome
Accession NZ_CP095856
Organism Escherichia coli strain EC6563

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag MYG04_RS20915 Protein ID WP_001291435.1
Coordinates 4333006..4333224 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag MYG04_RS20920 Protein ID WP_000344800.1
Coordinates 4333250..4333624 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MYG04_RS20880 (4328295) 4328295..4328867 + 573 WP_000779826.1 YbaY family lipoprotein -
MYG04_RS20885 (4328898) 4328898..4329209 - 312 WP_000409911.1 MGMT family protein -
MYG04_RS20895 (4329588) 4329588..4329941 + 354 WP_000878140.1 DUF1428 domain-containing protein -
MYG04_RS20900 (4329983) 4329983..4331533 - 1551 WP_108429885.1 cyclic-guanylate-specific phosphodiesterase PdeB -
MYG04_RS20905 (4331697) 4331697..4332167 - 471 WP_000136192.1 YlaC family protein -
MYG04_RS20910 (4332283) 4332283..4332834 - 552 WP_000102564.1 maltose O-acetyltransferase -
MYG04_RS20915 (4333006) 4333006..4333224 - 219 WP_001291435.1 HHA domain-containing protein Toxin
MYG04_RS20920 (4333250) 4333250..4333624 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
MYG04_RS20925 (4334170) 4334170..4337319 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
MYG04_RS20930 (4337342) 4337342..4338535 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T242650 WP_001291435.1 NZ_CP095856:c4333224-4333006 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T242650 NZ_OW849082:c4949836-4949462 [Citrobacter freundii]
ATGGTCAAAGGATCCGCGCTTTTTGATACCAACATCCTCATCGATCTGTTTAGTGGGAGAAATGAAGCAAAACTAGCGAT
AGAAACATGGCCGCCGCAGAATGCAATCAGCCTCATTACATGGATGGAGGTGATGGTGGGTGCAAAAAAATACCATCAGG
AAGCCCGTACTCGTGTTGCGCTAGGTGCTTTTAACGTGATCGGCGTTTCACAGGATATTGCTGAACGAAGCGTGAGTATC
AGACAGGAATATGGGATGAAATTGCCGGATGCCATTATTTTGGCAACAGCACAGATTCATCGTCTTACGCTGGTTACGCG
AAATACTAAGGATTTTGCAGGCCTATCCGGTGTGGTCACACCGTATACATTATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT242650 WP_000344800.1 NZ_CP095856:c4333624-4333250 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT242650 NZ_OW849082:c4950078-4949836 [Citrobacter freundii]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGATGAAGTGATTAAACGTCTTGATGATCTCAAGGT
GCAGCGCAACCTGCCGCGAGCAGAACTTTTACGGGAGGCCGTTGAACAGTATCTTGAGAGGCAGGATCAGGCTGAAACCA
CAATTTCACGTGCTCTTGGGCTGTGGCAAGGCTGTGAGGAAGATGGTGTTGAATACCAGCGTAAACTCCGTGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References