Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 4298695..4299532 Replicon chromosome
Accession NZ_CP095856
Organism Escherichia coli strain EC6563

Toxin (Protein)


Gene name itaT Uniprot ID A0A140NB16
Locus tag MYG04_RS20745 Protein ID WP_000227786.1
Coordinates 4298695..4299237 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag MYG04_RS20750 Protein ID WP_001297137.1
Coordinates 4299221..4299532 (-) Length 104 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MYG04_RS20725 (4294225) 4294225..4295136 - 912 WP_000705853.1 2-dehydropantoate 2-reductase -
MYG04_RS20730 (4295304) 4295304..4295795 + 492 WP_001138904.1 nucleotide binding protein YajQ -
MYG04_RS20735 (4295923) 4295923..4297287 - 1365 WP_001000978.1 MFS transporter -
MYG04_RS20740 (4297704) 4297704..4298639 + 936 WP_108429874.1 tetratricopeptide repeat protein -
MYG04_RS20745 (4298695) 4298695..4299237 - 543 WP_000227786.1 GNAT family N-acetyltransferase Toxin
MYG04_RS20750 (4299221) 4299221..4299532 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
MYG04_RS20755 (4299717) 4299717..4300607 - 891 WP_000971336.1 heme o synthase -
MYG04_RS20760 (4300619) 4300619..4300948 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
MYG04_RS20765 (4300948) 4300948..4301562 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
MYG04_RS20770 (4301552) 4301552..4303543 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
MYG04_RS20775 (4303565) 4303565..4304512 - 948 WP_001239440.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(68-156)

Antitoxin

(14-97)


Sequences


Toxin        


Download         Length: 181 a.a.        Molecular weight: 19794.98 Da        Isoelectric Point: 8.3395

>T242649 WP_000227786.1 NZ_CP095856:c4299237-4298695 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADSAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T242649 NZ_OW849082:4645183-4645563 [Citrobacter freundii]
ATGCAAACCTTATCCGCCATCCCGAAGCGGAAGGCACCGTCACGCCCGACGCCCGTTGAAATCTGGCAGCAACTTCTTAC
GTATCTGTTAAAAAGACATTACGGCCTTTCGCTTAGCGACACGCAATTTTCCGATGAGGAGATTATTACGCAATATATTG
ACGCCGGTATCTCACTGTCAGATGCACTCAATTTTCTCGTTGAAAAATCTGAACTGGTGCGGATTGATCGTCCCGGTTTC
TCCATTAAGCATCAGTCCCCTTTTATCGGGGTGATTGATATTCTCCGGGCCAGACGGGCAACGGGTTTGATGCAACGTAA
CGGGTATAAAAGGATCACTCTTCTCATCGCTGGAAATGCAGCTCAGGAGCAACATTCATGA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT242649 WP_001297137.1 NZ_CP095856:c4299532-4299221 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT242649 NZ_OW849082:4644760-4645125 [Citrobacter freundii]
TTGTCGAATCCAATACCTCCAGTAAACCATGATGTCTCTGAGCCCTGGTGGGGGCTGAAACCGGGCATCACTCCGTGTTT
TGGCGCACGACTGGTACAGGAAGGCAACCGCCTGCATTATCTGGCTGATCGGGCCAGTATCGCCGGTGTGTTCAGCGATG
CGGATTTACGCCATCTCGATCAAGCATTTCCGGTACTGCTCAAACAGCTGGAATTAATGCTGGTTTCCGGTGAGCTCAAT
CCCTGCCATCAGCACTGTGTCACGTTATATGCAAAAGGGCTGACCTGTGAAGCAGATTCCCTCGGATCACATGGCTACAT
TTATACAGCAATTTATCCGACACCAGACGACAGCATAACCCGGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A140NB16


Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References