Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 2150580..2151156 Replicon chromosome
Accession NZ_CP095843
Organism Escherichia fergusonii strain EF21QZZ116

Toxin (Protein)


Gene name shpA Uniprot ID A0A0G9FRD2
Locus tag MYF53_RS10545 Protein ID WP_001350372.1
Coordinates 2150869..2151156 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag MYF53_RS10540 Protein ID WP_000063156.1
Coordinates 2150580..2150882 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MYF53_RS10520 (2146394) 2146394..2146906 + 513 WP_001175455.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -
MYF53_RS10525 (2147176) 2147176..2149326 + 2151 WP_001314407.1 pyruvate/proton symporter BtsT -
MYF53_RS10530 (2149376) 2149376..2149579 + 204 WP_000467859.1 YbdD/YjiX family protein -
MYF53_RS10535 (2149590) 2149590..2150546 + 957 WP_001297640.1 GTPase -
MYF53_RS10540 (2150580) 2150580..2150882 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
MYF53_RS10545 (2150869) 2150869..2151156 - 288 WP_001350372.1 BrnT family toxin Toxin
MYF53_RS10550 (2151312) 2151312..2152046 - 735 WP_181665714.1 Fic family protein -
MYF53_RS10555 (2152309) 2152309..2155821 + 3513 WP_249896271.1 type I restriction-modification system endonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11209.68 Da        Isoelectric Point: 7.4020

>T242605 WP_001350372.1 NZ_CP095843:c2151156-2150869 [Escherichia fergusonii]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T242605 NZ_OW848981:33999-34415 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT242605 WP_000063156.1 NZ_CP095843:c2150882-2150580 [Escherichia fergusonii]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT242605 NZ_OW848981:33772-34002 [Escherichia coli]
ATGAGAACCGTATCGATATTCAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9FRD2

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References