Detailed information of TA system TA242592

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1241206..1242123
• Replicon: chromosome
• Accession: NZ_CP095842
• Organism: Bacillus velezensis strain EM-1

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: MYE78_RS06495
• Protein ID: WP_007407256.1
• Coordinates: 1241377..1242123 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: MYE78_RS06490
• Protein ID: WP_003154807.1
• Coordinates: 1241206..1241376 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYE78_RS06450	1236439..1238061	+	1623	WP_032874601.1	pyocin knob domain-containing protein	-
MYE78_RS06455	1238074..1238445	+	372	WP_032874603.1	XkdW family protein	-
MYE78_RS06460	1238450..1238647	+	198	WP_032874605.1	XkdX family protein	-
MYE78_RS06465	1238704..1239465	+	762	WP_032874607.1	hypothetical protein	-
MYE78_RS06470	1239517..1239780	+	264	WP_003154815.1	hemolysin XhlA family protein	-
MYE78_RS06475	1239794..1240057	+	264	WP_003154813.1	phage holin	-
MYE78_RS06480	1240071..1240949	+	879	WP_032874609.1	N-acetylmuramoyl-L-alanine amidase	-
MYE78_RS06485	1240984..1241109	-	126	WP_003154809.1	hypothetical protein	-
MYE78_RS06490	1241206..1241376	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
MYE78_RS06495	1241377..1242123	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
MYE78_RS06500	1242228..1243226	-	999	WP_032874611.1	inorganic phosphate transporter	-
MYE78_RS06505	1243239..1243856	-	618	WP_032874614.1	DUF47 domain-containing protein	-
MYE78_RS06510	1244142..1245458	-	1317	WP_032874616.1	amino acid permease	-
MYE78_RS06515	1245780..1246730	+	951	WP_032874618.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1197452..1299975	102523

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T242592 WP_007407256.1 NZ_CP095842:c1242123-1241377 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T242592 NZ_OW848980:1109515-1109826 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT242592 WP_003154807.1 NZ_CP095842:c1241376-1241206 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT242592 NZ_OW848980:1109823-1110242 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14