Detailed information of TA system TA024259

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 58375..59027
• Replicon: chromosome
• Accession: NC_013235
• Organism: Nakamurella multipartita DSM 44233

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: C8XHZ9
• Locus tag: NAMU_RS00275
• Protein ID: WP_012813965.1
• Coordinates: 58626..59027 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: C8XHZ8
• Locus tag: NAMU_RS00270
• Protein ID: WP_012813964.1
• Coordinates: 58375..58629 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NAMU_RS00245	53537..54397	+	861	WP_012813958.1	citryl-CoA lyase	-
NAMU_RS00250	54390..55253	+	864	WP_012813959.1	extradiol ring-cleavage dioxygenase	-
NAMU_RS00255	55255..55614	+	360	WP_012813960.1	hypothetical protein	-
NAMU_RS00260	55639..57132	+	1494	WP_052307719.1	amidohydrolase family protein	-
NAMU_RS00265	57350..58327	+	978	WP_012813963.1	zinc-binding alcohol dehydrogenase family protein	-
NAMU_RS00270	58375..58629	+	255	WP_012813964.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
NAMU_RS00275	58626..59027	+	402	WP_012813965.1	type II toxin-antitoxin system VapC family toxin	Toxin
NAMU_RS00280	59425..62850	+	3426	WP_012813966.1	DEAD/DEAH box helicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

• Antitoxin VapB-like (3-83)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 13702.44 Da
• Isoelectric Point: 4.4056

>T24259 WP_012813965.1 NC_013235:58626-59027 [Nakamurella multipartita DSM 44233]
VSPVVLDTSAVAAVIFGEPDAEAFAAALTRHGGELSISAVTRVELEIVLEARQGSEAVSDAEAMFDRLGVITEPVDAAQA
GAAFAAWRRFGKGRHPAGLNLGDCFSYALARAAGAPLLFKGNDFAQTDIASAL

Nucleotide

• Length: 402 bp

>T24259 NC_013235:58626-59027 [Nakamurella multipartita DSM 44233]
GTGAGTCCTGTCGTTCTCGACACCTCGGCCGTCGCCGCCGTGATCTTCGGTGAACCGGACGCCGAAGCCTTTGCCGCGGC
CCTGACGCGCCATGGCGGTGAACTCAGCATCAGCGCCGTCACCCGCGTCGAACTCGAGATCGTCCTCGAGGCGAGGCAGG
GATCCGAGGCGGTGTCCGACGCGGAGGCGATGTTCGACCGGCTGGGCGTCATCACCGAGCCGGTGGACGCCGCCCAGGCC
GGCGCGGCCTTCGCGGCGTGGCGGCGGTTCGGGAAGGGCCGCCATCCCGCCGGTCTGAACCTCGGCGACTGTTTCAGCTA
TGCGCTCGCCCGAGCGGCGGGGGCGCCGTTGCTGTTCAAGGGCAACGACTTCGCTCAGACCGATATCGCCAGCGCGCTGT
GA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9591.74 Da
• Isoelectric Point: 5.2536

>AT24259 WP_012813964.1 NC_013235:58375-58629 [Nakamurella multipartita DSM 44233]
VVLNIKDPETDRLARELAAEAGESLTVATRRAIEERLSRLRARKRANDPDLGLDEFIRRGRERAVLDERSPDDIIGYDEH
GLPR

Nucleotide

• Length: 255 bp

>AT24259 NC_013235:58375-58629 [Nakamurella multipartita DSM 44233]
GTGGTGTTGAACATCAAGGATCCGGAAACCGATCGCCTCGCGCGCGAATTGGCCGCCGAAGCTGGTGAATCGCTCACCGT
GGCCACCCGCCGGGCGATCGAGGAGCGACTGTCGCGTCTCCGCGCTCGGAAGCGCGCGAACGACCCCGATCTGGGTCTGG
ATGAGTTCATCCGACGAGGACGGGAACGTGCGGTGCTCGATGAGCGCTCGCCCGACGACATCATCGGGTACGACGAGCAC
GGCCTTCCTCGGTGA

Structures

Toxin

• AlphaFold DB: C8XHZ9

Antitoxin

• AlphaFold DB: C8XHZ8