Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 112791..113316 Replicon plasmid pJD053-144K
Accession NZ_CP095788
Organism Escherichia fergusonii strain EF21JD053

Toxin (Protein)


Gene name ccdB Uniprot ID F0JY91
Locus tag MYB88_RS22985 Protein ID WP_001159869.1
Coordinates 113011..113316 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag MYB88_RS22980 Protein ID WP_000813630.1
Coordinates 112791..113009 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MYB88_RS25570 108479..108629 - 151 Protein_116 hypothetical protein -
MYB88_RS22960 (MYB88_22955) 108582..108812 - 231 WP_252990174.1 IS66 family insertion sequence element accessory protein TnpB -
MYB88_RS22965 (MYB88_22960) 109690..110340 + 651 Protein_118 transposase -
MYB88_RS22970 (MYB88_22965) 110618..111214 - 597 Protein_119 IS66 family transposase -
MYB88_RS22975 (MYB88_22970) 111304..111732 - 429 Protein_120 hypothetical protein -
MYB88_RS22980 (MYB88_22975) 112791..113009 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
MYB88_RS22985 (MYB88_22980) 113011..113316 + 306 WP_001159869.1 type II toxin-antitoxin system toxin CcdB Toxin
MYB88_RS22990 (MYB88_22985) 113317..113517 + 201 Protein_123 phage integrase family protein -
MYB88_RS22995 (MYB88_22990) 113502..114413 - 912 WP_000549737.1 DMT family transporter -
MYB88_RS23000 (MYB88_22995) 114530..114667 + 138 Protein_125 Lrp/AsnC family transcriptional regulator -
MYB88_RS23005 (MYB88_23000) 114667..115716 + 1050 Protein_126 IS4 family transposase -
MYB88_RS23010 (MYB88_23005) 115816..117189 - 1374 WP_000244368.1 IS4-like element ISEc13 family transposase -
MYB88_RS23015 (MYB88_23010) 117401..117835 - 435 WP_000525034.1 IS200/IS605 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..144572 144572
- inside IScluster/Tn - - 107631..117835 10204


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11678.46 Da        Isoelectric Point: 6.4674

>T242583 WP_001159869.1 NZ_CP095788:113011-113316 [Escherichia fergusonii]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTSDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T242583 NZ_OW848788:c4891517-4891143 [Citrobacter freundii]
ATGGTCAAAGGATCCGCGCTTTTTGATACCAACATCCTCATCGATCTGTTTAGTGGGAGAAATGAAGCAAAACTAGCGAT
AGAAACATGGCCGCCGCAGAATGCAATCAGCCTCATTACATGGATGGAGGTGATGGTGGGTGCAAAAAAATACCATCAGG
AAGCCCGTACTCGTGTTGCGCTAGGTGCTTTTAACGTGATCGGCGTTTCACAGGATATTGCTGAACGAAGCGTGAGTATC
AGACAGGAATATGGGATGAAATTGCCGGATGCCATTATTTTGGCAACAGCACAGATTCATCGTCTTACGCTGGTTACGCG
AAATACTAAGGATTTTGCAGGCCTATCCGGTGTGGTCACACCGTATACATTATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT242583 WP_000813630.1 NZ_CP095788:112791-113009 [Escherichia fergusonii]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT242583 NZ_OW848788:c4891759-4891517 [Citrobacter freundii]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGATGAAGTGATTAAACGTCTTGATGATCTCAAGGT
GCAGCGCAACCTGCCGCGAGCAGAACTTTTACGGGAGGCCGTTGAACAGTATCTTGAGAGGCAGGATCAGGCTGAAACCA
CAATTTCACGTGCTCTTGGGCTGTGGCAAGGCTGTGAGGAAGATGGTGTTGAATACCAGCGTAAACTCCGTGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3V2SIB9

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References