Detailed information of TA system TA242582

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 64219..64820
• Replicon: plasmid pJD053-144K
• Accession: NZ_CP095788
• Organism: Escherichia fergusonii strain EF21JD053

Toxin (Protein)

• Gene name: doc
• Uniprot ID: V0AJ64
• Locus tag: MYB88_RS22660
• Protein ID: WP_001216034.1
• Coordinates: 64219..64599 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: MYB88_RS22665
• Protein ID: WP_001190712.1
• Coordinates: 64599..64820 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYB88_RS22635 (MYB88_22630)	59659..61143	-	1485	WP_000124159.1	hypothetical protein	-
MYB88_RS22640 (MYB88_22635)	61143..62336	-	1194	WP_000219605.1	terminase	-
MYB88_RS22645 (MYB88_22640)	62423..62875	-	453	WP_001312282.1	late promoter-activating protein (Gp10)	-
MYB88_RS22650 (MYB88_22645)	62964..64007	-	1044	WP_000648827.1	DUF968 domain-containing protein	-
MYB88_RS22655 (MYB88_22650)	64035..64214	-	180	WP_078182672.1	PdcA protein	-
MYB88_RS22660 (MYB88_22655)	64219..64599	-	381	WP_001216034.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
MYB88_RS22665 (MYB88_22660)	64599..64820	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MYB88_RS22670 (MYB88_22665)	64893..65282	-	390	WP_000506730.1	S24 family peptidase	-
MYB88_RS22675 (MYB88_22670)	65457..65813	+	357	WP_208634916.1	DNA-binding protein	-
MYB88_RS22680 (MYB88_22675)	65810..66028	+	219	WP_208634917.1	hypothetical protein	-
MYB88_RS22685 (MYB88_22680)	66029..66385	+	357	WP_001062545.1	hypothetical protein	-
MYB88_RS22690 (MYB88_22685)	67461..67823	-	363	WP_208634918.1	hypothetical protein	-
MYB88_RS22695 (MYB88_22690)	68666..68773	-	108	Protein_67	hypothetical protein	-
MYB88_RS22700 (MYB88_22695)	68984..69118	-	135	Protein_68	hypothetical protein	-
MYB88_RS22705 (MYB88_22700)	69115..69480	-	366	WP_001229294.1	HNH endonuclease signature motif containing protein	-
MYB88_RS22710 (MYB88_22705)	69482..69700	-	219	WP_114211923.1	DUF4014 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..144572	144572

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13615.32 Da
• Isoelectric Point: 5.1514

>T242582 WP_001216034.1 NZ_CP095788:c64599-64219 [Escherichia fergusonii]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T242582 NZ_OW848788:c4474690-4474406 [Citrobacter freundii]
ATGACTTATGAACTGGAATTCGACCCGAGAGCCTTAAAAGAGTGGCATAAACTGGGTGATACGGTGAAGGCTCAGTTTAA
GAAGAAGTTGGCCGACGTGTTGTTGAACCCCAGAATTGACTCTGCCCGTTTAAACGGTCTTCCAGATTGCTATAAAATTA
AGCTTAAATCGTCCGGCTATCGTCTGGTGTACCAGGTTCGGGATGAGGTTGTGACCGTGTTTGTTGTCGCGGTCGGCAAG
CGACAACATTCGGCCGTTTATCTCGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT242582 WP_001190712.1 NZ_CP095788:c64820-64599 [Escherichia fergusonii]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT242582 NZ_OW848788:c4474922-4474680 [Citrobacter freundii]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCTGTGCGATTACTGTTTCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACCGTTAGCGACA
GCGAAGATACACTGATTCAGACAGTCCGGGAGAGGCTGGCCAGCCCGCAAAAAGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• AlphaFold DB: V0AJ64

Antitoxin

• AlphaFold DB: A0A829CJB6