Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 85419..87042 Replicon plasmid pJD053-230K
Accession NZ_CP095787
Organism Escherichia fergusonii strain EF21JD053

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag MYB88_RS21545 Protein ID WP_000088046.1
Coordinates 85717..87042 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag MYB88_RS21540 Protein ID WP_024136327.1
Coordinates 85419..85712 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MYB88_RS21515 (MYB88_21510) 81291..82532 + 1242 WP_001053338.1 VWA domain-containing protein -
MYB88_RS25555 82623..82754 + 132 WP_000374059.1 hypothetical protein -
MYB88_RS21520 (MYB88_21515) 83095..83376 - 282 WP_000077926.1 hypothetical protein -
MYB88_RS21525 (MYB88_21520) 83426..83617 - 192 WP_000398480.1 hypothetical protein -
MYB88_RS21530 (MYB88_21525) 83709..84050 - 342 WP_001151575.1 hypothetical protein -
MYB88_RS21535 (MYB88_21530) 84423..84815 + 393 WP_000341066.1 hypothetical protein -
MYB88_RS21540 (MYB88_21535) 85419..85712 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
MYB88_RS21545 (MYB88_21540) 85717..87042 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
MYB88_RS21550 (MYB88_21545) 87103..87309 + 207 WP_000134171.1 hypothetical protein -
MYB88_RS21555 (MYB88_21550) 87411..87821 + 411 WP_000985911.1 hypothetical protein -
MYB88_RS21560 (MYB88_21555) 87834..88079 + 246 Protein_86 HNH endonuclease -
MYB88_RS21565 (MYB88_21560) 88132..88836 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
MYB88_RS21570 (MYB88_21565) 88950..89726 + 777 WP_001199192.1 aminoglycoside N-acetyltransferase AAC(3)-IVa -
MYB88_RS21575 (MYB88_21570) 89747..89968 + 222 WP_000171321.1 hypothetical protein -
MYB88_RS21580 (MYB88_21575) 89955..90980 + 1026 WP_000742814.1 aminoglycoside O-phosphotransferase APH(4)-Ia -
MYB88_RS21585 (MYB88_21580) 91055..91294 + 240 Protein_91 AAC(3) family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(3)-IVa / aph(4)-Ia / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / lnu(F) / ant(3'')-Ia / blaTEM-1B / tet(A) / qnrS1 / blaCTX-M-65 - 1..230045 230045


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T242581 WP_000088046.1 NZ_CP095787:85717-87042 [Escherichia fergusonii]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T242581 NZ_OW848788:c4337341-4337054 [Citrobacter freundii]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCACAAAGTAACCTGAGAAAACACGGTGTCCGGTTTGAGGATGCGGT
GCTGGTGTTTGACGATCCCCAACATCTTTCGCGGCAGGATCGGCATGAGAACGGTGAGTATCGCTGGCAGACCATCGGTC
TCGTTCATGGCATTGTGGTTATTCTGGTTGCCCATAGCGTTAGGTTTGAAAGTGGCTTTGAAGTCATTCGTATCATCAGT
GCCAGAAAGGCAGACAGTAAGGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT242581 WP_024136327.1 NZ_CP095787:85419-85712 [Escherichia fergusonii]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT242581 NZ_OW848788:c4337067-4336765 [Citrobacter freundii]
ATGAGCATGGTTAAGCATAAATGCGGCAGCGTATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAAGCGCTGGCGAA
TAAACCGGACGATGATATCGATTACAGTGACATTCCCGTGACCGAAGATAAGCAGTGGTCAGAGGCCACACGCGGTAAGT
TTTTCCGTCCGTTAAAAACGCAGGCCTCGGTGCGTATTGATGCCGATGTGATGGAGTGGCTGAAACGTCCAGGAAAAGGA
TATCAGACGCGCCTTAATGCCATTTTGCGCGAAGCGATGCTGCGCGAGCAAAATAAGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References