Detailed information of TA system TA024257

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3909628..3910236
• Replicon: chromosome
• Accession: NC_013216
• Organism: Desulfotomaculum acetoxidans DSM 771

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: C8VWT7
• Locus tag: DTOX_RS18170
• Protein ID: WP_015759135.1
• Coordinates: 3909628..3909978 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: DTOX_RS18175
• Protein ID: WP_042317449.1
• Coordinates: 3909982..3910236 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DTOX_RS18140	3905027..3905476	-	450	WP_015759129.1	ribosomal protein S18-alanine N-acetyltransferase	-
DTOX_RS18145	3905479..3906204	-	726	WP_015759130.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB	-
DTOX_RS18150	3906185..3906664	-	480	WP_015759131.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE	-
DTOX_RS18155	3906963..3907370	-	408	WP_015759132.1	response regulator	-
DTOX_RS18160	3907497..3908657	-	1161	WP_015759133.1	amidohydrolase	-
DTOX_RS18165	3908664..3909377	-	714	WP_015759134.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-
DTOX_RS18170	3909628..3909978	-	351	WP_015759135.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DTOX_RS18175	3909982..3910236	-	255	WP_042317449.1	ribbon-helix-helix protein, CopG family	Antitoxin
DTOX_RS18180	3910340..3910858	-	519	WP_015759137.1	acetolactate synthase small subunit	-
DTOX_RS18185	3911033..3912217	-	1185	WP_015759138.1	nucleotidyltransferase family protein	-
DTOX_RS18190	3912204..3913916	-	1713	WP_042316204.1	ABC transporter ATP-binding protein/permease	-
DTOX_RS18195	3913891..3914385	-	495	WP_015759140.1	S24/S26 family peptidase	-
DTOX_RS18200	3914378..3914641	-	264	WP_015759141.1	PqqD family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12611.72 Da
• Isoelectric Point: 5.6926

>T24257 WP_015759135.1 NC_013216:c3909978-3909628 [Desulfofarcimen acetoxidans DSM 771]
MLIRRGDIFFADLSPVIGSEQGGTRPVLILQNDIGNQYSPTTIVAAITSQIAKAKLPTHVEMEASACGLEKNSVVLIEQI
RTIDKSRLLEKVATLNDELMSKVNHAIEISMGLLPL

Nucleotide

• Length: 351 bp

>T24257 NC_013216:c3909978-3909628 [Desulfofarcimen acetoxidans DSM 771]
ATGCTGATCCGTCGCGGTGATATATTTTTTGCTGATTTGAGTCCTGTAATTGGTTCAGAGCAGGGTGGTACCAGGCCCGT
TCTGATTCTGCAAAACGATATAGGCAACCAGTATAGTCCGACTACTATTGTAGCGGCTATTACCTCGCAGATTGCTAAAG
CCAAGCTGCCTACGCATGTAGAGATGGAGGCTTCAGCCTGCGGGCTGGAAAAGAACTCAGTTGTTTTAATCGAACAGATT
AGGACAATTGATAAAAGCAGGCTTTTAGAAAAAGTTGCTACGTTAAATGATGAATTAATGAGTAAGGTTAATCATGCTAT
TGAAATAAGTATGGGTCTTTTACCTCTATGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9694.28 Da
• Isoelectric Point: 6.3609

>AT24257 WP_042317449.1 NC_013216:c3910236-3909982 [Desulfofarcimen acetoxidans DSM 771]
MISLPDSLLAEVDGIAAAERVNRSEFIREAMKLYIAERKRRLLREQMKKGYMEMANINLQLAVEQYRLETEVTPAFEAYL
TGAK

Nucleotide

• Length: 255 bp

>AT24257 NC_013216:c3910236-3909982 [Desulfofarcimen acetoxidans DSM 771]
ATGATAAGTCTGCCGGACAGTCTTTTAGCTGAGGTTGACGGCATAGCAGCTGCTGAAAGAGTAAACCGCAGCGAATTCAT
CAGGGAGGCTATGAAATTGTACATTGCCGAGCGCAAGCGCCGGTTGCTAAGAGAACAGATGAAAAAGGGATATATGGAAA
TGGCAAATATTAATCTTCAGCTTGCGGTGGAACAGTACCGTTTGGAGACGGAGGTTACACCGGCTTTTGAAGCATATTTG
ACGGGGGCAAAATAA

Structures

Toxin

• AlphaFold DB: C8VWT7