Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vagCD/VapC-VagC |
| Location | 17449..18092 | Replicon | plasmid p112Kb |
| Accession | NZ_CP095778 | ||
| Organism | Klebsiella pneumoniae strain bo714 | ||
Toxin (Protein)
| Gene name | vagD | Uniprot ID | D8L2J9 |
| Locus tag | MX575_RS28450 | Protein ID | WP_001044770.1 |
| Coordinates | 17676..18092 (+) | Length | 139 a.a. |
Antitoxin (Protein)
| Gene name | vagC | Uniprot ID | D5KTK7 |
| Locus tag | MX575_RS28445 | Protein ID | WP_001261282.1 |
| Coordinates | 17449..17679 (+) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MX575_RS28405 (MX575_28405) | 12647..13120 | + | 474 | WP_004152341.1 | YkgJ family cysteine cluster protein | - |
| MX575_RS28410 (MX575_28410) | 13212..13442 | + | 231 | WP_011977773.1 | hypothetical protein | - |
| MX575_RS28415 (MX575_28415) | 14334..15116 | - | 783 | WP_004152340.1 | site-specific integrase | - |
| MX575_RS28420 (MX575_28420) | 15116..15448 | - | 333 | WP_004152339.1 | hypothetical protein | - |
| MX575_RS28425 (MX575_28425) | 15455..15853 | - | 399 | WP_004171440.1 | hypothetical protein | - |
| MX575_RS28430 (MX575_28430) | 15879..16208 | - | 330 | WP_004152337.1 | hypothetical protein | - |
| MX575_RS28435 (MX575_28435) | 16236..16544 | - | 309 | WP_004152336.1 | hypothetical protein | - |
| MX575_RS28440 (MX575_28440) | 17031..17492 | - | 462 | WP_072093212.1 | hypothetical protein | - |
| MX575_RS28445 (MX575_28445) | 17449..17679 | + | 231 | WP_001261282.1 | type II toxin-antitoxin system VapB family antitoxin | Antitoxin |
| MX575_RS28450 (MX575_28450) | 17676..18092 | + | 417 | WP_001044770.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| MX575_RS28455 (MX575_28455) | 18166..18855 | + | 690 | Protein_19 | AAA family ATPase | - |
| MX575_RS28460 (MX575_28460) | 18910..19607 | + | 698 | WP_223174630.1 | IS1 family transposase | - |
| MX575_RS28465 (MX575_28465) | 19623..19733 | + | 111 | Protein_21 | mercuric transport protein periplasmic component | - |
| MX575_RS28470 (MX575_28470) | 19769..20190 | + | 422 | Protein_22 | organomercurial transporter MerC | - |
| MX575_RS28475 (MX575_28475) | 20242..21935 | + | 1694 | Protein_23 | mercury(II) reductase | - |
| MX575_RS28480 (MX575_28480) | 21953..22315 | + | 363 | WP_001277456.1 | mercury resistance co-regulator MerD | - |
| MX575_RS28485 (MX575_28485) | 22312..22548 | + | 237 | WP_000993386.1 | broad-spectrum mercury transporter MerE | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | blaTEM-1A / blaOXA-9 / blaKPC-3 | - | 1..112243 | 112243 | |
| - | flank | IS/Tn | - | - | 11259..12527 | 1268 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 139 a.a. Molecular weight: 15106.52 Da Isoelectric Point: 7.1084
>T242553 WP_001044770.1 NZ_CP095778:17676-18092 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR
Download Length: 417 bp
>T242553 NZ_OW848785:c2031207-2030893 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8756.83 Da Isoelectric Point: 4.4692
>AT242553 WP_001261282.1 NZ_CP095778:17449-17679 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL
Download Length: 231 bp
>AT242553 NZ_OW848785:c2030896-2030480 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0H3GYM2 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0H3GZG3 |