242552

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 141179..141915 Replicon plasmid p363Kb
Accession NZ_CP095777
Organism Klebsiella pneumoniae strain bo714

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag MX575_RS27195 Protein ID WP_003026803.1
Coordinates 141179..141661 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag MX575_RS27200 Protein ID WP_003026799.1
Coordinates 141649..141915 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MX575_RS27175 (MX575_27175) 136341..136829 + 489 WP_004152114.1 phosphate-starvation-inducible PsiE family protein -
MX575_RS27180 (MX575_27180) 136816..137778 + 963 WP_004152113.1 zinc metalloprotease HtpX -
MX575_RS27185 (MX575_27185) 137955..139120 + 1166 Protein_166 IS3 family transposase -
MX575_RS29375 139329..139460 - 132 WP_004218042.1 hypothetical protein -
MX575_RS27190 (MX575_27190) 139621..140967 - 1347 WP_020314316.1 ISNCY family transposase -
MX575_RS27195 (MX575_27195) 141179..141661 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
MX575_RS27200 (MX575_27200) 141649..141915 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
MX575_RS27205 (MX575_27205) 142091..142345 - 255 WP_004152108.1 hypothetical protein -
MX575_RS27210 (MX575_27210) 142421..142678 - 258 WP_004152107.1 hypothetical protein -
MX575_RS27215 (MX575_27215) 142727..142930 - 204 WP_004152106.1 HHA domain-containing protein -
MX575_RS27220 (MX575_27220) 142964..143332 - 369 WP_004152105.1 hypothetical protein -
MX575_RS27225 (MX575_27225) 143376..143870 - 495 WP_004152104.1 DNA-binding protein -
MX575_RS27230 (MX575_27230) 143901..144476 - 576 WP_004152103.1 hypothetical protein -
MX575_RS27235 (MX575_27235) 144464..144733 - 270 WP_004152102.1 hypothetical protein -
MX575_RS27240 (MX575_27240) 145091..145441 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
MX575_RS27245 (MX575_27245) 145491..145853 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid floR / tet(A) / aph(6)-Id / aph(3'')-Ib / aph(3')-VIb / sul2 / blaCMY-16 / aph(3')-Ia / aac(6')-Ib / catA1 / dfrA14 / ARR-3 / cmlA1 / blaOXA-10 / ant(3'')-Ia / qacE / sul1 - 1..363560 363560


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T242552 WP_003026803.1 NZ_CP095777:c141661-141179 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T242552 NZ_OW848785:1852208-1852585 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCAGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT242552 WP_003026799.1 NZ_CP095777:c141915-141649 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT242552 NZ_OW848785:1851751-1852119 [Escherichia coli]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCTCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCCTGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCGGATGCGTACCATCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACCGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References