Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5270873..5271498 Replicon chromosome
Accession NZ_CP095776
Organism Klebsiella pneumoniae strain bo714

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag MX575_RS25990 Protein ID WP_002882817.1
Coordinates 5270873..5271256 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag MX575_RS25995 Protein ID WP_004150355.1
Coordinates 5271256..5271498 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MX575_RS25975 (MX575_25975) 5268239..5269141 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
MX575_RS25980 (MX575_25980) 5269138..5269773 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
MX575_RS25985 (MX575_25985) 5269770..5270699 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
MX575_RS25990 (MX575_25990) 5270873..5271256 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
MX575_RS25995 (MX575_25995) 5271256..5271498 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
MX575_RS26000 (MX575_26000) 5271703..5272620 + 918 WP_002882812.1 alpha/beta hydrolase -
MX575_RS26005 (MX575_26005) 5272634..5273575 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
MX575_RS26010 (MX575_26010) 5273620..5274057 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
MX575_RS26015 (MX575_26015) 5274054..5274914 - 861 WP_002882807.1 virulence factor BrkB family protein -
MX575_RS26020 (MX575_26020) 5274908..5275507 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T242550 WP_002882817.1 NZ_CP095776:c5271256-5270873 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T242550 NZ_OW848785:c787683-787432 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT242550 WP_004150355.1 NZ_CP095776:c5271498-5271256 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT242550 NZ_OW848785:c787435-787181 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References