Detailed information of TA system TA242549

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4946733..4948414
• Replicon: chromosome
• Accession: NZ_CP095776
• Organism: Klebsiella pneumoniae strain bo714

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: W8V1Q6
• Locus tag: MX575_RS24490
• Protein ID: WP_002885155.1
• Coordinates: 4946733..4947650 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: W8UPQ8
• Locus tag: MX575_RS24495
• Protein ID: WP_004151732.1
• Coordinates: 4947656..4948414 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MX575_RS24470 (MX575_24470)	4942039..4943559	+	1521	WP_002885173.1	sugar ABC transporter ATP-binding protein	-
MX575_RS24475 (MX575_24475)	4943556..4944554	+	999	WP_002885172.1	ribose ABC transporter permease	-
MX575_RS24480 (MX575_24480)	4944585..4945526	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
MX575_RS24485 (MX575_24485)	4945601..4946608	+	1008	WP_004151731.1	carbohydrate kinase family protein	-
MX575_RS24490 (MX575_24490)	4946733..4947650	-	918	WP_002885155.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
MX575_RS24495 (MX575_24495)	4947656..4948414	-	759	WP_004151732.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
MX575_RS24500 (MX575_24500)	4948500..4949228	-	729	WP_002885152.1	response regulator transcription factor	-
MX575_RS24505 (MX575_24505)	4949501..4951648	+	2148	WP_077598858.1	formate dehydrogenase subunit alpha	-
MX575_RS24510 (MX575_24510)	4951878..4952564	+	687	WP_004151733.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-246)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34687.15 Da
• Isoelectric Point: 5.4707

>T242549 WP_002885155.1 NZ_CP095776:c4947650-4946733 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIVYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T242549 NZ_OW848785:c787435-787181 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28564.92 Da
• Isoelectric Point: 10.0870

>AT242549 WP_004151732.1 NZ_CP095776:c4948414-4947656 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLSFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT242549 NZ_OW848785:c787683-787432 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GH11

Antitoxin

• AlphaFold DB: A0A0H3GQB5