Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4687268..4688078 Replicon chromosome
Accession NZ_CP095776
Organism Klebsiella pneumoniae strain bo714

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag MX575_RS23190 Protein ID WP_002887280.1
Coordinates 4687268..4687801 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag MX575_RS23195 Protein ID WP_002887278.1
Coordinates 4687812..4688078 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MX575_RS23185 (MX575_23185) 4686099..4687220 + 1122 WP_002887282.1 cupin domain-containing protein -
MX575_RS23190 (MX575_23190) 4687268..4687801 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
MX575_RS23195 (MX575_23195) 4687812..4688078 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
MX575_RS23200 (MX575_23200) 4688181..4689614 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
MX575_RS23205 (MX575_23205) 4689604..4690287 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
MX575_RS23210 (MX575_23210) 4690459..4691844 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
MX575_RS23215 (MX575_23215) 4691862..4692206 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T242547 WP_002887280.1 NZ_CP095776:c4687801-4687268 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T242547 NZ_OW848785:c515777-515675 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT242547 WP_002887278.1 NZ_CP095776:c4688078-4687812 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT242547 NZ_OW848785:515637-515905 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATATTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References